Dataset Information

MassSpecPreppy - an end-to-end solution for automated protein concentration determination and flexible sample digestion for proteomics applications

ABSTRACT: In proteomics, fast, efficient and highly reproducible sample preparation are of utmost importance, particularly in view of fast scanning mass spectrometers enabling analyses of large sample series. To address this need, we have developed the web application MassSpecPreppy that operates on the open science OT-2 liquid handling robot from Opentrons. This platform can prepare up to 96 samples at once, performing tasks like BCA protein concentration determination, sample digestion with normalization, reduction/alkylation, enzymatic digestion, and peptide elution or loading specified peptide amounts onto Evotips in an automated and flexible manner.

INSTRUMENT(S): timsTOF Pro 2, Orbitrap Exploris 480

ORGANISM(S): Saccharomyces Cerevisiae Strain C13-aby.s86 Escherichia Coli K-12 (ncbitaxon:83333) Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Stephan Michalik

PROVIDER: MSV000092489 | MassIVE | Thu Jul 20 04:19:00 BST 2023

REPOSITORIES: MassIVE

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MassSpecPreppy-An end-to-end solution for automated protein concentration determination and flexible sample digestion for proteomics applications.

Reder Alexander A Hentschker Christian C Steil Leif L Gesell Salazar Manuela M Hammer Elke E Dhople Vishnu M VM Sura Thomas T Lissner Ulrike U Wolfgramm Hannes H Dittmar Denise D Harms Marco M Surmann Kristin K Völker Uwe U Michalik Stephan S

Proteomics 20230929 9

In proteomics, fast, efficient, and highly reproducible sample preparation is of utmost importance, particularly in view of fast scanning mass spectrometers enabling analyses of large sample series. To address this need, we have developed the web application MassSpecPreppy that operates on the open science OT-2 liquid handling robot from Opentrons. This platform can prepare up to 96 samples at once, performing tasks like BCA protein concentration determination, sample digestion with normalizatio ...[more]

PMID: 37772677

Dataset Information

MassSpecPreppy - an end-to-end solution for automated protein concentration determination and flexible sample digestion for proteomics applications

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MassSpecPreppy-An end-to-end solution for automated protein concentration determination and flexible sample digestion for proteomics applications.

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