Proteomics

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Systematic analysis of proteome turnover in an organoid model of pancreatic cancer by dSILO


ABSTRACT: The role of protein turnover in pancreatic ductal adenocarcinoma (PDA) metastasis has not previously been investigated. We introduce dynamic Stable-Isotope Labeling of Organoids (dSILO): a dynamic SILAC derivative that combines a pulse of isotopically labeled amino acids with isobaric tandem-mass tag (TMT) labeling to measure proteome-wide protein turnover rates in organoid systems.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Mus Musculus (ncbitaxon:10090)

SUBMITTER: Marko Jovanovic   Christine Chio  

PROVIDER: MSV000092498 | MassIVE | Fri Jul 21 13:10:00 BST 2023

REPOSITORIES: MassIVE

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