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The maintenance of oocytes in the mammalian ovary involves extreme protein longevity

ABSTRACT: In mammals, oocytes are formed in the female embryo and need to be preserved in the ovary to ensure the viability of the next generation. How oocytes are maintained for decades is unclear. Here, we combined pulse-chase stable isotope labelling coupled with mass spectrometry, single-cell RNA-seq, microscopy and NanoSims to create an atlas of protein homeostasis in mouse oocytes and ovaries over the entire reproductive lifespan. Our results show that protein turnover in the ovary is much slower than in other organs, with hundreds of extremely long-lived proteins across a broad range of complexes and pathways, including mitochondria, ribosomes and the cytoskeleton. We propose that slow protein turnover helps to maintain proteostasis in oocytes and the ovary over long periods, protecting the germline across generations.

INSTRUMENT(S): Orbitrap Fusion, Orbitrap Exploris 480

ORGANISM(S): Mus Musculus (ncbitaxon:10090)

SUBMITTER: Dr. Juliane Liepe Prof. Henning Urlaub Dr. Melina Schuh

PROVIDER: MSV000092528 | MassIVE | Wed Jul 26 10:52:00 BST 2023

SECONDARY ACCESSION(S): PXD044113

REPOSITORIES: MassIVE

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