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Multi-Protease Investigation of Arabidopsis Thaliana Cell Culture Mitochondria

ABSTRACT: The aim of this project is to deeply map the proteome of mitochondria from the model plant Arabidopsis thaliana. For this purpose, mitochondria were isolated from Arabidopsis cell cultures, their proteins extracted and processed using SP3 digestion. To achieve high sequence coverage, the proteins were digested with a total of six different proteases and measured using sensitive timsTOF Pro hardware and TIMS fractionation.

INSTRUMENT(S): timsTOF Pro

ORGANISM(S): Arabidopsis Thaliana (ncbitaxon:3702)

SUBMITTER: Hans-Peter Braun Nils Rugen

PROVIDER: MSV000092578 | MassIVE | Wed Aug 02 23:21:00 BST 2023

SECONDARY ACCESSION(S): PXD044312

REPOSITORIES: MassIVE

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Deep proteomics reveals incorporation of unedited proteins into mitochondrial protein complexes in Arabidopsis.

Rugen Nils N Senkler Michael M Braun Hans-Peter HP

Plant physiology 20240501 2

The mitochondrial proteome consists of numerous types of proteins which either are encoded and synthesized in the mitochondria, or encoded in the cell nucleus, synthesized in the cytoplasm and imported into the mitochondria. Their synthesis in the mitochondria, but not in the nucleus, relies on the editing of the primary transcripts of their genes at defined sites. Here, we present an in-depth investigation of the mitochondrial proteome of Arabidopsis (Arabidopsis thaliana) and a public online p ...[more]

PMID: 38060994

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