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Metabolomics of novel Actinobacteria from oligotrophic subsurface soils

ABSTRACT: There is a growing interest in the role of metabolite exchange (cross-feeding) in microbial community stability, resilience and longitudinal dynamics. Though the number of genome sequences available to researchers continues to grow, we still have a poor understanding of how to relate genomic information to exometabolite production/consumption capacity and how that capacity changes across different conditions. Our experimental design will be to obtain polar metabolomes and transcriptomes simultaneously from three conditions for each strain: Condition 1) mid-logarithmic phase (control); Condition 2) trace metal limited stationary phase; and Condition 3) nitrogen limited stationary phase. These experimental conditions were chosen because the bioavailability of trace metals and nitrogen in subsurface environments can be variable and nutrient availability has been shown to influence exometabolite profiles of Actinobacteria (Senges, et al 2018 PNAS). The work (proposal:https://doi.org/10.46936/10.25585/60001177) conducted by the U.S. Department of Energy Joint Genome Institute (https://ror.org/04xm1d337), a DOE Office of Science User Facility, is supported by the Office of Science of the U.S. Department of Energy operated under Contract No. DE-AC02-05CH11231.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Actinobacteria

SUBMITTER: Paul Carini

PROVIDER: MSV000092601 | MassIVE | Fri Aug 04 13:22:00 BST 2023

REPOSITORIES: MassIVE

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