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Data files for manuscript "Acidic methanol treatment facilitates MALDI-mass spectrometry imaging of energy metabolism"

ABSTRACT: The MALDI mass spectrometry image files for mouse brain sections under various wash condition in negative mode with NEDC matrix and positive mode with THAP matrix: no wash control, wash with 5 mL chloroform, 5 mL MeOH, and 5mL MeOH + 0.05%FA (Fig 1c, Fig S7, Table S7). The files were converted from Bruker .d files to reduce file size and can be opened using IsoScope (https://github.com/xxing9703/Isoscope) under MATLAB environment. Spatial resolution is 50 um.

INSTRUMENT(S): solariX

ORGANISM(S): Mus Musculus (ncbitaxon:10090)

SUBMITTER: Wenyun Lu Shawn M. Davidson

PROVIDER: MSV000092611 | MassIVE | Mon Aug 07 08:03:00 BST 2023

REPOSITORIES: MassIVE

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Publications

Acidic Methanol Treatment Facilitates Matrix-Assisted Laser Desorption Ionization-Mass Spectrometry Imaging of Energy Metabolism.

Lu Wenyun W Park Noel R NR TeSlaa Tara T Jankowski Connor S R CSR Samarah Laith L McReynolds Melanie M Xing Xi X Schembri Jessica J Woolf Morgan T MT Rabinowitz Joshua D JD Davidson Shawn M SM

Analytical chemistry 20230927 40

Detection of small molecule metabolites (SMM), particularly those involved in energy metabolism using MALDI-mass spectrometry imaging (MSI), is challenging due to factors including ion suppression from other analytes present (e.g., proteins and lipids). One potential solution to enhance SMM detection is to remove analytes that cause ion suppression from tissue sections before matrix deposition through solvent washes. Here, we systematically investigated solvent treatment conditions to improve SM ...[more]

PMID: 37756255

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