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Parallel host and microbial pathways regulate lipid desaturation

ABSTRACT: 4 datasets, each in their own subfolder. 1) acdh-11 comparative metabolomics. 4 genotypes (N2, fcmt-1, WT FAT-7::GFP, and acdh-11;FAT-7::GFP) grown at 3 temperatures (15, 20, 25). Conditioned media (exo-metabolome) and worm pellet (endo-metabolome) isolated and extracted separately. 2) acdh-11 comparative metabolomics re: diet. 2 genotypes (WT FAT-7::GFP, and acdh-11;FAT-7::GFP) and 2 diets (WT E. coli, BW25113, and cyclopropane-deficient E. Coli, JW1653-1). The cyclopropane-deficient strain was additionally supplemented with cyclopropane fatty acids lactobacillic acid (LBA) or dihydrosterculic acid (DHSA) at 20 uM. 3) fcmt-1 comparative metabolomics. 4 genotypes (N2, fcmt-1(gk155709), fcmt-1(tm2382), hacl-1(tm6725)) grown at 20C. Conditioned media (exo-metabolome) and worm pellet (endo-metabolome) isolated and extracted separately. 4) vaccenic acid isotope labeling. 2 genotypes (N2 and hacl-1(tm6725)) grown at 20C. Each genotype was supplemented with vehicle, cis-vaccenic acid (cis-VA), stable isotope labeled D13-cis-VA, trans-vaccenic acid (trans-VA), and stable isotope labeled D13-trans-VA. Conditioned media (exo-metabolome) and worm pellet (endo-metabolome) isolated and extracted separately.

INSTRUMENT(S): Q Exactive HF, Q Exactive

ORGANISM(S): Caenorhabditis Elegans (ncbitaxon:6239)

SUBMITTER: Frank Schroeder

PROVIDER: MSV000092700 | MassIVE | Sun Aug 20 07:50:00 BST 2023

REPOSITORIES: MassIVE

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GNPS - Parallel host and microbial pathways regulate lipid desaturation

Project description:4 datasets, each in their own subfolder. 1) acdh-11 comparative metabolomics. 4 genotypes (N2, fcmt-1, WT FAT-7::GFP, and acdh-11;FAT-7::GFP) grown at 3 temperatures (15, 20, 25). Conditioned media (exo-metabolome) and worm pellet (endo-metabolome) isolated and extracted separately. 2) acdh-11 comparative metabolomics re: diet. 2 genotypes (WT FAT-7::GFP, and acdh-11;FAT-7::GFP) and 2 diets (WT E. coli, BW25113, and cyclopropane-deficient E. Coli, JW1653-1). The cyclopropane-deficient strain was additionally supplemented with cyclopropane fatty acids lactobacillic acid (LBA) or dihydrosterculic acid (DHSA) at 20 uM. 3) fcmt-1 comparative metabolomics. 4 genotypes (N2, fcmt-1(gk155709), fcmt-1(tm2382), hacl-1(tm6725)) grown at 20C. Conditioned media (exo-metabolome) and worm pellet (endo-metabolome) isolated and extracted separately. 4) vaccenic acid isotope labeling. 2 genotypes (N2 and hacl-1(tm6725)) grown at 20C. Each genotype was supplemented with vehicle, cis-vaccenic acid (cis-VA), stable isotope labeled D13-cis-VA, trans-vaccenic acid (trans-VA), and stable isotope labeled D13-trans-VA. Conditioned media (exo-metabolome) and worm pellet (endo-metabolome) isolated and extracted separately.

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