Dataset Information

Proteomic dataset of exosomes derived from fetal mesenchymal stromal cells

ABSTRACT: The raw files of the proteomics dataset are N=18 and a brief description of the files is shown below: AF1 raw: exosomes derived from Amniotic Fluid-Mesenchymal Stromal cells 1 AF2.raw: exosomes derived from Amniotic Fluid-Mesenchymal Stromal cells 2 AFA1.raw: exosomes derived from Amniotic Fluid-Mesenchymal Stromal cells 3 AFA2.raw: exosomes derived from Amniotic Fluid-Mesenchymal Stromal cells 4 AFB1.raw: exosomes derived from Amniotic Fluid-Mesenchymal Stromal cells 5 AFB2.raw: exosomes derived from Amniotic Fluid-Mesenchymal Stromal cells 6 HL1A.raw: exosomes derived from Hepatocyte like cells 1 HL1B.raw: exosomes derived from Hepatocyte like cells 2 HL2A.raw: exosomes derived from Hepatocyte like cells 3 HL2B.raw: exosomes derived from Hepatocyte like cells 4 HL3A.raw: exosomes derived from Hepatocyte like cells 5 HL3B.raw: exosomes derived from Hepatocyte like cells 6 HPLA1.raw: exosomes derived from Hepatic Progenitor-like cells 1 HPLA2.raw: exosomes derived from Hepatic Progenitor-like cells 2 HPLB1.raw: exosomes derived from Hepatic Progenitor-like cells 3 HPLB2.raw: exosomes derived from Hepatic Progenitor-like cells 4 HPLA.raw: exosomes derived from Hepatic Progenitor-like cells 5 HPLB.raw: exosomes derived from Hepatic Progenitor-like cells 6 Raw files analysis was performed with Proteome Discoverer 1.4 (Thermo) software package, using the Sequest search engine and the Uniprot human (Homo sapiens) reviewed database, downloaded on December 15, 2017, including 20,243 entries. The search was performed using carbamidomethylation of cysteine as static and oxidation of methionine as dynamic modifications. Two missed cleavage sites, a precursor mass tolerance of 10 ppm and fragment mass tolerance of 0.05 Da were allowed. False discovery rate (FDR) validation was based on q value: target FDR : 0.05. Label free quantification was performed by utilizing the precursor ion area values exported from the total ion chromatogram as defined by the Proteome Discoverer v. 1.4.0.288 (Thermo Scientific). Output files from Proteome Discoverer were processed with R programming language for statistical computing (version 4.0.3). The following comparisons were made: AF vs HL, AF vs HPL and HL vs HPL. The msf files are N=18 and have the same name as the raw files above.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Maria (Maya) Roubelakis

PROVIDER: MSV000092848 | MassIVE |

SECONDARY ACCESSION(S): PXD045348

REPOSITORIES: MassIVE

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Publications

MFGE-8 identified in fetal mesenchymal-stromal-cell-derived exosomes ameliorates acute hepatic failure pathology.

Psaraki Adriana A Zagoura Dimitra D Ntari Lydia L Makridakis Manousos M Nikokiraki Christina C Trohatou Ourania O Georgila Konstantina K Karakostas Christos C Angelioudaki Ioanna I Kriebardis Anastasios G AG Gramignioli Roberto R Sakellariou Stratigoula S Xilouri Maria M Eliopoulos Aristides G AG Vlahou Antonia A Roubelakis Maria G MG

iScience 20230930 11

Liver transplantation is the gold-standard therapy for acute hepatic failure (AHF) with limitations related to organ shortage and life-long immunosuppressive therapy. Cell therapy emerges as a promising alternative to transplantation. We have previously shown that IL-10 and Annexin-A1 released by amniotic fluid human mesenchymal stromal cells (AF-MSCs) and their hepatocyte progenitor-like (HPL) or hepatocyte-like (HPL) cells induce liver repair and downregulate systemic inflammation in a CCl<sub ...[more]

PMID: 37915594

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