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Analysis of cardiac ischemia-reperfusion injury using DIA proteomics and N-terminomics

ABSTRACT: Data used in the publication: Analysis of cardiac ischemia-reperfusion injury using DIA proteomics and N-terminomics reveals alterations in mitochondrial function and metabolism

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Rattus (ncbitaxon:10114)

SUBMITTER: Olivier Julien

PROVIDER: MSV000092906 | MassIVE |

REPOSITORIES: MassIVE

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Publications

Data-Independent Acquisition Proteomics and N-Terminomics Methods Reveal Alterations in Mitochondrial Function and Metabolism in Ischemic-Reperfused Hearts.

Hartley Bridgette B Bassiouni Wesam W Roczkowsky Andrej A Fahlman Richard R Schulz Richard R Julien Olivier O

Journal of proteome research 20240124 2

Myocardial ischemia-reperfusion (IR) (stunning) injury triggers changes in the proteome and degradome of the heart. Here, we utilize quantitative proteomics and comprehensive degradomics to investigate the molecular mechanisms of IR injury in isolated rat hearts. The control group underwent aerobic perfusion, while the IR injury group underwent 20 min of ischemia and 30 min of reperfusion to induce a stunning injury. As MMP-2 activation has been shown to contribute to myocardial injury, hearts a ...[more]

PMID: 38264990

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