Dataset Information

Jatropha curcas root and leaf metabolomic analysis

ABSTRACT: This work present a large data set related to a non-target LC-MS/MS high-resolution metabolome analysis of the leaves and roots of Jatropha curcas. Besides its high protein contents, the seeds of J. curcas are also rich in oil, so the seeds are considered a potential source of raw material to produce biodiesel and a source of food for farm animals. The full exploitation of these potentials is hampered by the presence of a highly toxic class of diterpenoids, the phorbol esters (PE), both in the oil and in the seedcake derived from the extraction of the oil. The seeds are known to possess 6 different PE, and although their structures have been known for almost 30 years, the biosynthetic pathway of each one remains undetermined. However, there are indications that the enzyme casbene synthase is responsible for the first biosynthetic step. The leaves and roots of this species are also rich in several classes of diterpenes which by themselves have pharmacological potential not yet explored. For our analysis, we used two genotypes that are contrasting regarding the contents of phorbol esters: One genotype has a high level of phorbol esters (HPE), while the other has low levels of phorbol esters in the seeds (LPE). We were able to detect a total of 2032 features and applied multivariate statistical methods, such as PCA and heatmap with hierarchical clustering to analyze and visualize the deposition pattern of metabolites, with a focus on terpenoids. Our analysis allowed us to several new findings regarding the synthesis of PE in J. curcas, such as that only in HPE genotypes roots are found diterpenes with the tigliane skeleton. In contrast, the LPE genotype has a higher concentration and diversity of sesquiterpenes. Taking into consideration the results of our previous proteome analysis of seeds, roots, and leaves and the metabolome data presented in the manuscript, we suggest that the absence of PE in the LPE genotype may be the result of the absence of the enzymes responsible for the final biosynthetic steps, which in turn lead to the accumulation of sesquiterpenes this genotype.

INSTRUMENT(S): Q Exactive Plus

ORGANISM(S): Jatropha Curcas (ncbitaxon:180498)

SUBMITTER: Domingos F. M. Neto

PROVIDER: MSV000092920 | MassIVE |

REPOSITORIES: MassIVE

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Project description:This work present a large data set related to a non-target LC-MS/MS high-resolution metabolome analysis of the leaves and roots of Jatropha curcas. Besides its high protein contents, the seeds of J. curcas are also rich in oil, so the seeds are considered a potential source of raw material to produce biodiesel and a source of food for farm animals. The full exploitation of these potentials is hampered by the presence of a highly toxic class of diterpenoids, the phorbol esters (PE), both in the oil and in the seedcake derived from the extraction of the oil. The seeds are known to possess 6 different PE, and although their structures have been known for almost 30 years, the biosynthetic pathway of each one remains undetermined. However, there are indications that the enzyme casbene synthase is responsible for the first biosynthetic step. The leaves and roots of this species are also rich in several classes of diterpenes which by themselves have pharmacological potential not yet explored. For our analysis, we used two genotypes that are contrasting regarding the contents of phorbol esters: One genotype has a high level of phorbol esters (HPE), while the other has low levels of phorbol esters in the seeds (LPE). We were able to detect a total of 2032 features and applied multivariate statistical methods, such as PCA and heatmap with hierarchical clustering to analyze and visualize the deposition pattern of metabolites, with a focus on terpenoids. Our analysis allowed us to several new findings regarding the synthesis of PE in J. curcas, such as that only in HPE genotypes roots are found diterpenes with the tigliane skeleton. In contrast, the LPE genotype has a higher concentration and diversity of sesquiterpenes. Taking into consideration the results of our previous proteome analysis of seeds, roots, and leaves and the metabolome data presented in the manuscript, we suggest that the absence of PE in the LPE genotype may be the result of the absence of the enzymes responsible for the final biosynthetic steps, which in turn lead to the accumulation of sesquiterpenes this genotype.

Project description:Background: Soil salinity is a major abiotic stress factor that limit agricultural productivity worldwide, and this problem is expected to grow in the future. Common bean (Phaseolus vulgaris L.) is an important protein source in developing countries is highly susceptible to salt stress. To understand the underlying mechanism of salt stress responses, transcriptomics, metabolomics, and ion content analysis were utilized for response comparison of salt-tolerant and salt-susceptible common bean genotypes in saline conditions. Results: Transcriptome analysis has revealed that the tolerant genotype had increased photosynthesis in saline conditions while the susceptible genotype acted in a contrasting way. The chlorophyll content measurements have backed up this result with increase in tolerant and decrease in susceptible genotype. Transcriptome also displayed a more active carbon and amino acid metabolism for the tolerant genotype as well. Analysis of primary metabolites with GC-MS demonstrated the boosted carbohydrate metabolism in the tolerant genotype with increased sugar content as well as better amino-acid metabolism with the accumulation of glutamate and asparagine and hinted a lowered photorespiration level for the tolerant one. Accumulation of lysine, valine, and isoleucine in the roots of the susceptible genotype suggested a halted stress response pathway. According to ion content comparison, the tolerant genotype managed to block accumulation of Na+ in the leaves while accumulating significantly less Na+ in the roots compared to susceptible genotype. K+ levels increased in the leaves of both genotype and the roots of the susceptible one but dropped in the roots of the tolerant genotype. Additionally, Zn+2 and Mn+2 levels were also dropped in the tolerant roots, while Mo+2 levels were significantly higher in all tissues in both control and saline conditions for tolerant genotype. Conclusion:The results of the presented study have demonstrated the differences in contrasting genotypes and thus provide valuable information on the pivotal molecular mechanisms underlying salt tolerance mainly in common bean, but for all crops.

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Jatropha curcas root and leaf metabolomic analysis

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