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MALDI mass spectrometry imaging of c-di-GMP in bacterial biofilms

ABSTRACT: Bacterial biofilms of V. cholerae, V. fischeri, and P. aeruginosa grown on agar we analyzed by MALDI mass spectrometry imaging in negative mode for the detection of cyclic dimeric guanosine monophosphate (c-di-GMP). P. aeruginosa samples were also analyzed in positive mode for the detection of additional metabolites. The presence of c-di-GMP was confirmed by MALDI MSMS of a V. cholerae extract. All raw data files are provided, .imzml datafiles provided as supplementary files.

INSTRUMENT(S): timsTOF fleX

ORGANISM(S): Pseudomonas Aeruginosa Pa14 (ncbitaxon:652611) Vibrio Cholerae O1 Biovar El Tor (ncbitaxon:686) Vibrio Fischeri Es114 (ncbitaxon:312309)

SUBMITTER: Laura Sanchez

PROVIDER: MSV000092966 | MassIVE |

REPOSITORIES: MassIVE

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GNPS - MALDI mass spectrometry imaging of c-di-GMP in bacterial biofilms

Project description:Bacterial biofilms of V. cholerae, V. fischeri, and P. aeruginosa grown on agar we analyzed by MALDI mass spectrometry imaging in negative mode for the detection of cyclic dimeric guanosine monophosphate (c-di-GMP). P. aeruginosa samples were also analyzed in positive mode for the detection of additional metabolites. The presence of c-di-GMP was confirmed by MALDI MSMS of a V. cholerae extract. All raw data files are provided, .imzml datafiles provided as supplementary files.

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