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The effect of abiotic stress on rhizosphere microbiota

ABSTRACT: The ultimate aim is see if rhizosphere microbiota are influenced by changes in root exudate composition resulting from abiotic stress. The abiotic variables we are focusing on at this stage are salinity, temperature and pH. This can be divided into two questions: (a) how do plant exudates change in response to abiotic stress, and (b) how do these changes influence bacteria. In order to test this we will produce plant exudates under controlled stressed conditions, measure their composition and measure bacterial growth in these exudates. Data has also been produced from synthetic community experiments comparing the community composition under a variety of controlled stress conditions (temperature, salinity, pH, and phosphate). The work (proposal:https://doi.org/10.46936/10.25585/60000944) conducted by the U.S. Department of Energy Joint Genome Institute (https://ror.org/04xm1d337), a DOE Office of Science User Facility, is supported by the Office of Science of the U.S. Department of Energy operated under Contract No. DE-AC02-05CH11231.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Microbiome

SUBMITTER: Jeff Dangl

PROVIDER: MSV000093257 | MassIVE | Thu Nov 02 10:19:00 GMT 2023

REPOSITORIES: MassIVE

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The effect of abiotic stress on rhizosphere microbiota

Project description:The ultimate aim is see if rhizosphere microbiota are influenced by changes in root exudate composition resulting from abiotic stress. The abiotic variables we are focusing on at this stage are salinity, temperature and pH. This can be divided into two questions: (a) how do plant exudates change in response to abiotic stress, and (b) how do these changes influence bacteria. In order to test this we will produce plant exudates under controlled stressed conditions, measure their composition and measure bacterial growth in these exudates. Data has also been produced from synthetic community experiments comparing the community composition under a variety of controlled stress conditions (temperature, salinity, pH, and phosphate). The work (proposal:https://doi.org/10.46936/10.25585/60000944) conducted by the U.S. Department of Energy Joint Genome Institute (https://ror.org/04xm1d337), a DOE Office of Science User Facility, is supported by the Office of Science of the U.S. Department of Energy operated under Contract No. DE-AC02-05CH11231.

2023-11-02 | MSV000093248 | MassIVE

Leveraging pan genomes to investigate diel transcriptomic and metabolomic responses to abiotic stress in Brassica. rapa and B. napus diversity panels.

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GNPS - Leveraging pan genomes to investigate diel transcriptomic and metabolomic responses to abiotic stress in Brassica. rapa and B. napus diversity panels.

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High resolution survey of drought, high salinity, heat and cold stress-induced transcriptome of bioenergy feedstock model plant western poplar (Populus trichocarpa) accession Nisqually-1

Project description:Abiotic stress is a major factor affecting the growth, development, yield and quality in plants including the important biomass yield such as in a bioenergy feedstock crops. In a first of its kind, RNA-seq based high resolution survey of abiotic stress-induced transcriptome of bioenergy feedstock model plant western poplar (Populus trichocarpa accesion Nisqually-1) was carried out by way of 81 libraries made from total RNA isolated from three tissues, mature vascular leaf, stem xylem and root sampled from plants subjected to untreated control and treated with four individual stress treatments cold, heat, drought and high salinity. For every tissue and treatment type including controls, three individual plants were used per treatment as biological replicates. This research project is supported by the DOE Office of Science, Office of Biological and Environmental Research (BER), USA, grant no. DE-SC0008570

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Exploring the role of drought-induced plant-associated microbes in promoting plant fitness in Sorghum bicolor and Oryza sativa

Project description:We investigate the role of root-associated Actinobacteria in promoting host fitness under drought stress in two plants important to the DOE mission of sustainable biofuels: Sorghum bicolor and Oryza sativa. The work (proposal:https://doi.org/10.46936/10.25585/60001083) conducted by the U.S. Department of Energy Joint Genome Institute (https://ror.org/04xm1d337), a DOE Office of Science User Facility, is supported by the Office of Science of the U.S. Department of Energy operated under Contract No. DE-AC02-05CH11231.

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Project description:We are examining the metabolites produced during co-culture of Xylariaceae fungi. The work (proposal:https://doi.org/10.46936/10.25585/60001144) conducted by the U.S. Department of Energy Joint Genome Institute (https://ror.org/04xm1d337), a DOE Office of Science User Facility, is supported by the Office of Science of the U.S. Department of Energy operated under Contract No. DE-AC02-05CH11231.

2023-11-02 | MSV000093277 | MassIVE

Expression data for stress treatment in rice seedlings

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Global metabolic profile comparison between 2 ecotypes upon inoculation with Azoarcus olearius

Project description:Arabidopsis thaliana associated with Plant Growth Promoting Bacteria (PGPB) Azoarcus olearius (DQS4) innoculated with WS (Wassilewskija) and Col0 (Columbia) ecotypes. The work (proposal:https://doi.org/10.46936/10.25585/60000448) conducted by the U.S. Department of Energy Joint Genome Institute (https://ror.org/04xm1d337), a DOE Office of Science User Facility, is supported by the Office of Science of the U.S. Department of Energy operated under Contract No. DE-AC02-05CH11231.

2023-11-02 | MSV000093274 | MassIVE

Genome-wide Gene Expression Profiling of Salinity Responsiveness in Rice

Project description:Abiotic stresses such as salinity are very important factors limiting rice growth and productivity around the world. Affymetrix rice genome array containing 48,564 japonica and 1,260 indica sequences was used to analyze the gene expression pattern of rice responsive to salinity stress, try to elucidate the difference of genome-wide gene expression profiling of two contrasting rice genotypes in response to salt stress and to discover the salinity related genes and gene interaction and networks. Under salinity condition, the number of differentially expressed genes (DEGs) in 177-103 was more than that in IR64, and most of up-regulated DEGs in 177-103 are response to stress. But in IR64, most of up-regulated DEGs are transcription related genes. The DEGs under salinity showed very strong tissue specificity, the number of DEGs in leaf was more than that in root. A lot of genes differentially expressed by exogenous ABA treatment under salinity condition, such as Leaf senescence protein, 1-deoxy-D-xylulose 5-phosphate synthase 2 precursor and Protein of unknown function DUF26 were induced by ABA and contributed to salinity tolerance.

2015-11-02 | GSE58603 | GEO

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