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Phosphoproteomics of dried blood collected on Mitra devices

ABSTRACT: Venous blood was collected in Li-heparin or EDTA blood tubes and loaded onto 20 uL Mitra devices or separated to plasma as detailed in metadata. Samples were denatured in 5.5% SDC, reduced and alkylated, and digested with TPCK-trypsin followed by acid precipitation. 1 mg of filtered precipitates were enriched for phosphopeptides using TiO. Data was acquied using nanoLC, Evosep One or SPE-ZipChip. Data was acquired by DIA (Exploris 480 or TimsTOF Pro 2) or LFQ-DDA. DIA data was analyzed with Spectronaut 18 and DDA with Skyline.

INSTRUMENT(S): timsTOF Pro 2, Orbitrap Exploris 480, Orbitrap Exploris 240

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Matthew W. Foster

PROVIDER: MSV000093624 | MassIVE | Sun Dec 10 07:42:00 GMT 2023

SECONDARY ACCESSION(S): PXD047676

REPOSITORIES: MassIVE

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