Genome-guided isolation of Fervidibacter sacchari, an aerobic, hyperthermophilic polysaccharide-degrading specialist
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ABSTRACT: Few aerobic hyperthermophiles degrade polysaccharides. Here, we describe the genome-enabled enrichment and optical tweezer-based isolation of an aerobic hyperthermophile, Fervidibacter sacchari, which was originally ascribed to candidate phylum Fervidibacteria. F. sacchari uses polysaccharides and monosaccharides as sole carbon sources from 65-87.5 Celsius and expresses 190 carbohydrate-active enzymes according to RNA-Seq and proteomics, including 31 with unusual glycoside hydrolase 177 or 179 domains.
INSTRUMENT(S): timsTOF Pro
ORGANISM(S): Fervidibacter Sacchari
SUBMITTER: Brian P. Hedlund
PROVIDER: MSV000093641 | MassIVE | Mon Dec 11 18:25:00 GMT 2023
SECONDARY ACCESSION(S): PXD047732
REPOSITORIES: MassIVE
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