Proteomics

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Global phosphoproteomics for CRISPR/Cas9 mediated PTPN22 Ser325 mutagenesis on Jurkat cells

ABSTRACT: LQF of 3x FLAG PTPN22 WT Jurkat cells compared to S325A and S325E KI mutant Jurkat cells. WT or KI lines were stimulated with or without TCR engagement for 5 min.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Nunzio Bottini   David J Gonzalez  

PROVIDER: MSV000093718 | MassIVE | Thu Dec 21 18:41:00 GMT 2023

SECONDARY ACCESSION(S): PXD048064

REPOSITORIES: MassIVE

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Protein tyrosine phosphatase nonreceptor type 22 (PTPN22) is encoded by a major autoimmunity gene and is a known inhibitor of T cell receptor (TCR) signaling and drug target for cancer immunotherapy. However, little is known about PTPN22 posttranslational regulation. Here, we characterize a phosphorylation site at Ser<sup>325</sup> situated C terminal to the catalytic domain of PTPN22 and its roles in altering protein function. In human T cells, Ser<sup>325</sup> is phosphorylated by glycogen sy  ...[more]

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