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Uncovering missing glycans and unexpected fragments with pGlycoNovo: a full-range Y-ion dynamic searching strategy for site-specific glycosylation analysis across species

ABSTRACT: Precision mapping of glycans at the site-specific level using mass spectrometry data has emerged as a crucial approach for glycan discovery in modern glycoproteomics and glycobiology. However, the extensive diversity of glycan compositions within and across species far surpasses the capacity of existing software databases. Consequently, the identification of glycans not included in the database or lacking prior compositional knowledge during large-scale glycoproteomic analyses poses a significant challenge. Here, we present pGlycoNovo, a software platform for analyzing intact glycopeptides featuring rare glycan attachments within the pGlyco3 software environment.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Drosophila <fruit Fly, Genus> (ncbitaxon:7215) Arabidopsis Thaliana (ncbitaxon:3702) Danio Rerio (ncbitaxon:7955) Mus Musculus (ncbitaxon:10090) Caenorhabditis Elegans (ncbitaxon:6239)

SUBMITTER: Weiqian Cao

PROVIDER: MSV000093838 | MassIVE | Thu Jan 11 11:20:00 GMT 2024

SECONDARY ACCESSION(S): PXD048469

REPOSITORIES: MassIVE

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