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Single Cell Proteoform Analysis Using scPiMS

ABSTRACT: We introduce single-cell Proteoform imaging Mass Spectrometry (scPiMS), which realizes the benefit of direct solvent extraction and MS detection of intact proteins from single cells drop casted onto glass slides. Sampling and detection of whole proteoforms by individual ion mass spectrometry enables a scalable approach to single-cell proteomics. This new scPiMS platform addresses the throughput bottleneck in single cell proteomics and boosts cell processing rate by several fold while accessing protein composition with higher coverage.

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Mus Musculus (ncbitaxon:10090)

SUBMITTER: Neil Kelleher

PROVIDER: MSV000093994 | MassIVE | Fri Feb 02 15:41:00 GMT 2024

REPOSITORIES: MassIVE

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Publications

Single Cell Analysis of Proteoforms.

Su Pei P Hollas Michael A R MAR Butun Fatma Ayaloglu FA Kanchustambham Vijaya Lakshmi VL Rubakhin Stanislav S Ramani Namrata N Greer Joseph B JB Early Bryan P BP Fellers Ryan T RT Caldwell Michael A MA Sweedler Jonathan V JV Kafader Jared O JO Kelleher Neil L NL

Journal of proteome research 20240318 6

We introduce single cell Proteoform imaging Mass Spectrometry (<i>scPiMS</i>), which realizes the benefit of direct solvent extraction and MS detection of intact proteins from single cells dropcast onto glass slides. Sampling and detection of whole proteoforms by individual ion mass spectrometry enable a scalable approach to single cell proteomics. This new <i>scPiMS</i> platform addresses the throughput bottleneck in single cell proteomics and boosts the cell processing rate by several fold whi ...[more]

PMID: 38497708

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