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Untargeted Metabolomics with E.coli and S.elongatus PCC 7942 before and after heat treatment

ABSTRACT: E.coli and Synechococcus elongatus PCC 7942 WT and a Mutant werde cultivated under standard growth conditions (37 or 28 degree C) and then heat shocked in a heat bath for 15 min at 58 degree C. (OD600 0.6 or 0.750 0.5). 50 mL of cells were harvested before and after treatment. Cell pellets were extracted with 20% or 80% MeOH. n=5. Column: Kinetex C18, 1.7 um EVO C18 100A, 50x2.1 mm. 500uL, 10min, 5-99%, DDA, Top5

INSTRUMENT(S): Q Exactive

ORGANISM(S): Synechococcus Elongatus Pcc 7942 (ncbitaxon:1140) Escherichia Coli (ncbitaxon:562)

SUBMITTER: Daniel Petras

PROVIDER: MSV000094005 | MassIVE | Sun Feb 04 23:30:00 GMT 2024

REPOSITORIES: MassIVE

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