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Molecular and Cellular Mechanisms of Teneurin Signaling in Synaptic Partner Matching

ABSTRACT: In the developing brain, axons exhibit remarkable precision in selecting synaptic partners among many non-partner cells. Teneurins are evolutionarily conserved transmembrane proteins that instruct synaptic partner matching via matched expression and homophilic attraction between synaptic partners. Little is known how intracellular signaling pathways execute this and diverse other functions triggered by extracellular interactions of teneurins. Here, we use in situ proximity labeling to identify Ten-ms intracellular interactome in the Drosophila brain. Genetic interaction using quantitative partner matching assays in both olfactory receptor neurons (ORNs) and projection neurons (PNs) suggest a common pathway Ten-m binds to and negatively regulates a RhoGAP, thus activating the Rac1 small GTPases to promote synaptic partner matching. Developmental analyses with single-axon resolution further reveal that ORN axons initially extend exuberant branches along their trajectory, and those that contact partner PN dendrites are selectively stabilized, accompanied by an increase of local F-actin accumulation.

INSTRUMENT(S): Q Exactive Plus

ORGANISM(S): Drosophila <fruit Fly, Genus> (ncbitaxon:7215)

SUBMITTER: Steven A. Carr

PROVIDER: MSV000094010 | MassIVE | Mon Feb 05 08:27:00 GMT 2024

REPOSITORIES: MassIVE

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