Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Methylobacterium sp. Leaf119 LC-MS Data

ABSTRACT: Raw LC-MS/MS data of crude extract of Methylobacterium sp. Leaf119. Included are .mzml and .raw files for a 12C-methanol blank, 13C-methanol blank, 13C-methanol plus 12C-PABA, 13C-methanol plus methionine, and 13C-methanol plus both PABA and methionine.

INSTRUMENT(S): Xevo G2-S QTof

ORGANISM(S): Methylobacterium Sp. Leaf119 (ncbitaxon:1736261)

SUBMITTER: Aaron Puri

PROVIDER: MSV000094181 | MassIVE | Tue Feb 27 09:58:00 GMT 2024

REPOSITORIES: MassIVE

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Methylobacterium sp. Leaf119 LC-MS Data

Project description:Raw LC-MS/MS data of crude extract of Methylobacterium sp. Leaf119. Included are .mzml and .raw files for a 12C-methanol blank, 13C-methanol blank, 13C-methanol plus 12C-PABA, 13C-methanol plus methionine, 13C-methanol plus both PABA and methionine, and 12C-methanol plus deuterated methionine (methyl-D3).

2024-04-30 | MSV000094644 | MassIVE

GNPS - Methylobacterium sp. Leaf119 LC-MS Data

2024-04-30 | MSV000094644 | GNPS

GNPS - Methylobacterium sp. Leaf119 LC-MS Data

2024-02-27 | MSV000094181 | GNPS

GroEL-proteotypbing-based stable isotope probing of Thauera aromatica K172, Pseudomonas putida KT2440, and Escherichia coli BL21(DE3)

Project description:This dataset has been used to establish GroEL-SIP, coupling a targeted proteotyping approach for assessing bacterial community compositions using the taxonomic marker protein GroEL with stable isotope probing to link the identified taxa to substrate assimilation. This dataset contains raw data of four experiments: 1.) Pure cultures of T. aromatica cultivated with 13C or 12C benzoate mixed in defined ratios. 2.) Pure cultures of P. putida cultivated with 13C or 12C benzoate mixed in a 1:1 ratio. 3.) Pure cultures of E. coli cultivated with 12C acetate and 13C or 12C benzoate mixed in a 1:1 ratio. 4.) Co-cultivated biculture of T. aromatica and P. putida cultivated with 13C or 12C benzoate and mixed in a 1:1 ratio. 5.) Co-cultivated biculture of T. aromatica and E. coli cultivated with 12c acetate and 13C or 12C benzoate and mixed in a 1:1 ratio. 1)-3) were analyzed after in-solution digestion 4)-5) were analyzed after in-gel digestion of the 60 kDa band

2024-10-17 | PXD048255 | Pride

Multi-omics Analysis of Salmonella enterica Serovar Typhimurium Infection of Wild Type C57BL/6 and Immunodeficient gp91-/-phox Mice

Project description:Three groups of sex- and aged-matched 9-12 week old C57BL/6 wild type mice and gp91-/-phox mice were infected by i.v. injection of bacterial suspensions: Group 1 represents wild type C57BL/6 mice infected with virulent S. Typhimurium SL1344 grown in vitro. (Raw files: OTNCS_AGSL1344#3C#9C#10C-XX) Group 2 represents wild type C57BL/6 mice infected with virulent S. Typhimurium SL1344 grown in vivo for 72 h in the Group 1 C57BL/6 mice. (Raw files: OTNCS_AGSL1344#9F#10F-XX) Group 3 represents immunodeficient gp91-/-phox mice infected with virulent S. Typhimurium SL1344 grown in vitro. (Raw files: OTNCS_AGSL1344#11C#12C#13C-XX) And Input (Raw files: OTNCS_AGSL1344mixedAa-XX and OTNCS_AGSL1344mixedAb-XX)

2018-10-26 | PXD005855 | Pride

GeoChip 5.0 microarray of the active bacterial community from both warming and control soil samples

Project description:To determine whether and how warming affects the functional capacities of the active microbial communities, GeoChip 5.0 microarray was used. Briefly, four fractions of each 13C-straw sample were selected and regarded as representative for the active bacterial community if 16S rRNA genes of the corresponding 12C-straw samples at the same density fraction were close to zero.

2023-12-30 | E-MTAB-13326 | biostudies-arrayexpress

Identification of targets of the protein disulfide reductase thioredoxin

Project description:Identification of targets of the protein disulfide reductase thioredoxin using liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) and thiol specific differential labeling with isotope-coded affinity tags (ICAT). Reduction of specific target disulfides is quantified by measuring ratios of cysteine residues labeled with the heavy (13C) and light (12C) ICAT reagents in peptides derived from tryptic digests of Trx-treated and non-treated samples. Keywords: protein, LC-MS/MS, ICAT

2008-11-20 | GSE11650 | GEO

Core metabolism shifts of methanol vs. methane growth in the methanotroph Methylomicrobium buryatense 5GB1

Project description:Methylomicrobium buryatense 5GB1 is an obligate methylotroph, which grows on methane or methanol with similar growth rates. Core metabolic pathways are similar on both substrates, but recent studies of methane metabolism suggest that growth on methanol might have significant differences from growth on methane. In this study, both a targeted metabolomics approach as well as a 13C tracer approach have been taken to understand core carbon metabolism in M. buryatense 5GB1 during methanol growth, to determine whether such differences occur. Targeted metabolomics analyses were performed on both methane and methanol cultures to identify metabolic nodes with altered fluxes. Several key metabolites showed significant differences in pool size. Noticeably, 2-keto-3-deoxy-6-phosphogluconate (KDPG) showed much larger pools under methanol culture, suggesting the Entner-Doudoroff (ED) pathway was more active. Intermediates in other parts of metabolism also showed differences in pool sizes under methanol growth. A systematic shift of active core metabolism is proposed to explain the changes. In order to distinguish flux partition differences at the C3-C4 node, 13C tracer analysis was also applied to methanol-grown cultures. Using the experimental results as constraints, we applied flux balance analysis to determine the metabolic flux phenotype of M. buryatense 5GB1 growing on methanol. The resulting new insights into core metabolism of this methanotroph provide an improved basis for future strain design.

2019-04-17 | GSE110541 | GEO

marine metagenome

Project description:13C methanol SIP (WCO station L4)

| PRJNA261236 | ENA

2023 Jardine - Populus Trichocarpa 13CO2 labeling experiment - C1 metabolism

Project description:A 13CO2 labelling experiment was performed on Populus trichocarpa using a branch enclosure for studying diurnal emissions of both 12C-methanol and 13C-methanol. Branches were supplied with 900 ppm of 13CO2. Controls in a 12C02 environment were included. After 2.5 days, treated and control leaves were removed from the plant, flash frozen under liquid nitrogen, and stored at -80C. Frozen tissues were homogenized by bead milling and extracted in methanol prior to LC-MS/MS.

2024-01-03 | MSV000093760 | MassIVE

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data