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Anti-Ferroptotic Effect of Cannabidiol in Human Skin Keratinocytes Characterized by Data-Independent Acquisition-Based Proteomics

ABSTRACT: SWATH-MS data for human keratinocytes (HaCaT cell line), sample 010-012 indicates HaCaT cells with no treatment, sample 013-015 indicates HaCaT cells induced by erastin, sample 0016-018 indicates HaCaT cells treated by erastin and CBD. For more detailed information, please contact Dr.Chang Liu (hichang813@uri.edu)

INSTRUMENT(S): TripleTOF 5600

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Navindra Seeram

PROVIDER: MSV000094348 | MassIVE | Mon Mar 18 12:53:00 GMT 2024

REPOSITORIES: MassIVE

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Anti-Ferroptotic Effect of Cannabidiol in Human Skin Keratinocytes Characterized by Data-Independent Acquisition-Based Proteomics.

Li Huifang H Puopolo Tess T Seeram Navindra P NP Liu Chang C Ma Hang H

Journal of natural products 20240219 5

Skin cells are susceptible to oxidative stress and various types of cell death, including an iron-dependent form known as ferroptosis. Cannabidiol (CBD) can protect skin cells against oxidative stress, but whether this is attributed to the inhibition of ferroptosis is unknown. Herein, we evaluated the anti-ferroptotic effect of CBD in human keratinocytes using biochemical assays (radical scavenging and iron chelating) and cell-based models (for lipid peroxidation and intracellular iron). CBD's a ...[more]

PMID: 38373879

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SWATH data for human keratinocytes treated with erastin and reactive carbonyl species

Project description:SWATH-MS data for human keratinocytes (HaCaT cell line), sample 001-004 indicates HaCaT cells with no treatment, sample 005-008 indicates HaCaT cells induced by erastin, sample 009-012 indicates HaCaT cells induced by erastin plus MGO (methyglyoxal), and sample 013-016 indicates HaCaT cells induced by erastin plus GO (glyoxal). For more detailed information, please contact Dr.Chang Liu (hichang813@uri.edu)

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