Proteomics

Dataset Information

0

Deep, Unbiased and Quantitative mass spectrometry-based plasma proteome analysis of personalized response to mRNA COVID-19 vaccine


ABSTRACT: Global efforts against COVID-19 have vaccinated a significant portion of the world population in recent years. Combating the COVID-19 pandemic with mRNA vaccines playinged a pivotal role in global immunization effort. However, individual responses to these vaccines vary, leading to diverse vaccination efficacy. Despite significant progress, full understanding of the molecular mechanisms driving the personalized immune response to COVID-19 vaccine remains elusive. To address this gap, we combined a novel nanoparticle-based proteomic workflow with tandem mass tag (TMT) labeling, to quantitatively assess the proteomic changes in a cohort of 12 volunteers following two doses of the Pfizer-BioNTech mRNA COVID-19 vaccine. This optimized protocol seamlessly integrates comprehensive proteome analysis with enhanced throughput by leveraging the enrichment of low-abundant plasma proteins by engineered nanoparticles. Our data demonstrate the ability of this nanoparticle-based workflow to quantify over 3,000 proteins from 48 human plasma samples, providing the deepest view into COVID-19 vaccine-related plasma proteome study. We identified 69 proteins exhibiting a boosted response to the vaccine after the second dose. Additionally, 74 proteins were differentially regulated between seven volunteers, who contracted COVID-19 despite receiving two doses of the vaccine, and the ones who did not contract COVID-19. These findings offer valuable insights into individual variability in response to vaccination, demonstrating the potential of personalized medicine approaches in vaccine development.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Alex Rosa Campos  

PROVIDER: MSV000094352 | MassIVE | Mon Mar 18 18:53:00 GMT 2024

SECONDARY ACCESSION(S): PXD050749

REPOSITORIES: MassIVE

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

2023-03-01 | PXD036608 | Pride
2024-04-01 | GSE249050 | GEO
2024-09-02 | BIOMD0000000978 | BioModels
2025-01-14 | GSE281864 | GEO
2022-12-09 | GSE190001 | GEO
2023-11-21 | GSE247910 | GEO
2023-11-21 | GSE247905 | GEO
2023-11-21 | GSE247904 | GEO
2023-11-21 | GSE247914 | GEO
2023-11-21 | GSE247913 | GEO