Project description:Here we used functional proteomics approaches to identify an upstream transcription complex (USTC) that is required for piRNA biogenesis. The complex contains PRDE-1, SNPC-4, TOFU-4, and TOFU-5, all of which are enriched on the two piRNA clusters on chromosome IV and form distinct piRNA foci in the nucleus.
Project description:Pseudouridine (Ψ) is the most abundant RNA modification in nature; however, the exact biological functions of Ψ remain largely elusive. By employing an unbiased quantitative proteomics method, we identified multiple candidate reader proteins of Ψ in RNA, including a cytoskeleton protein profilin-1, whose mutations are linked with amyotrophic lateral sclerosis (ALS). We purified recombinant PFN1 and showed that it can bind directly and selectively to Ψ-containing RNA. We also found that PFN1 binds to thousands of transcripts in human cells, including a known Ψ site in the mRNA of TPI1. We showed that PFN1-Ψ interaction is crucial for regulating the stability and translation efficiency of TPI1 mRNA. Together, our data unveiled PFN1 as a reader protein of Ψ in RNA and illustrated the functions of PFN1-Ψ interaction in modulating the stability and translation efficiency of TPI1 mRNA, which provides new insights into the functions of Ψ in RNA biology and lays a strong foundation for the discovery of new mechanisms in disease pathogenesis
Project description:This project contains data of RP-LC-MS lipidomics of protein complex pull-downs (CoQ7, CoQ9, and CoQ7:CoQ9); data are associated with the manuscript titled "Structure and functionality of a multimeric human COQ7:COQ9 complex".
Project description:Lipidomics, proteomics and metabolomics characterization of the ontogeny of lipid, protein and metabolite changes during normal postnatal lung development
Project description:Piwi-interacting RNAs (piRNAs) play significant roles in suppressing transposons and nonself nucleic acids, maintaining genome integrity, and defending against viral infections, and are essential for fertility in a variety of organisms. In C. elegans, most piRNA precursors are transcribed by RNA polymerase II in the nucleus and are subjected to a number of processing and maturation steps. However, the biogenesis of piRNAs is still not fully understood. We used functional proteomics to study piRNA biogenesis in C. elegans and identified a piRNA processing and chromosome segregation (PICS) complex. The PICS complex contains two known piRNA biogenesis factors, TOFU-6 and PID-1, and three new proteins PICS-1, TOST-1, and ERH-2, which exhibit dynamic localization among different subcellular compartments. In the germlines of gravid animals, the PICS complex contains TOFU-6/PICS-1/ERH-2/PID-1, is largely concentrated at the perinuclear granule zone and engages in piRNA processing. During early embryogenesis, the TOFU-6/PICS-1/ERH-2/TOST-1 complex accumulates in the nucleus and plays essential roles in chromosome segregation and cell division. Interestingly, the functions of these factors in mediating chromosome segregation are independent of piRNA production. Therefore, we speculate that differential compositions of PICS factors may help cells coordinate distinct cellular processes.
Project description:Interventions: Nutrition intervention group 1:Perioperative oral general sugar clear liquid diet (200 ml/bag) at time points;Nutrition intervention group 2:Perioperative Oral Complex Carbohydrate Purified Liquid Food (200ml/bag);Blank control group 3:Fasting from food and water 12 hours before surgery and 24 hours after surgery
Primary outcome(s): Intestinal barrier function;Semiquantitative typing of intestinal flora;Gut flora 16sRNA assay;albumin;Length of stay in hospital;Hospitalisation costs
Study Design: Parallel