Distinct proteomic brain states underlying long-term memory formation in aversive operant conditioning
Ontology highlight
ABSTRACT:
INSTRUMENT(S): Q Exactive HF-X
ORGANISM(S): Lymnaea Stagnalis (ncbitaxon:6523)
SUBMITTER: 
Zhong-Ping Feng
PROVIDER: MSV000094487 | MassIVE | Mon Apr 08 06:45:00 BST 2024
REPOSITORIES: MassIVE
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Our hypotheses are 1) the long-lasting memory in 7 h-trained males is accompanied with characteristic temporal changes in gene expression in the brain, 2) per mutant males are defective in regulation of critical genes involved in long-term courtship memory, 3) the memoryârelated changes in gene expression can be detected by comparing total head transcript levels between trained and naïve as well as the wild type and the per mutant males using the Affymetrix microarray. Two fly strains, a wild type Oregon R (OR) and a null mutant allele of per (per01) with the OR background for the second and third chromosomes, will be used. Virgin males and females will be collected without anesthesia within 6 hrs after eclosion and maintained individually in vials until experiments. Mated wild type females will be prepared by crossing to the wild type males the night before they are used for conditioning males. All flies will be raised at 25oC in a 12 h light/12 h dark cycle and the following behavioral experiments will be carried out during daytime. For courtship conditioning, a 3 day-old virgin male (either OR or per01) will be placed with a mated OR female in a conditioning chamber (15 mm x 5 mm in depth) containing fly food. As a control, a virgin male will be introduced into another conditioning chamber without a mated female. Seven hours after the male is introduced into the chamber, he will be anesthetized under CO2 gas or transferred to a new vial. For the anesthetized male, the head will be immediately excised and frozen with liquid nitrogen. The male fly transferred to a new vial will be decapitated 24 hrs after the end of the 7-hr training. Approximately thirty fly heads will be pooled for each of the 8-genotype/treatment groups, i.e., Oregon R 0-hr and 24-hr after conditioning, per01 0-hr and 24-hr after conditioning, in addition to the corresponding four naïve controls. 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