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Proteomic analysis of unicellular cyanobacterium Crocosphaera subtropica ATCC 51142 under extended light or dark growth

ABSTRACT: The daily light-dark cycle is a recurrent and predictable environmental phenomenon to which many organisms, including cyanobacteria, have evolved to adapt. Understanding how cyanobacteria alter their metabolic attributes in response to subjective light or dark growth may provide key features for developing strains with an improved photosynthetic efficiency as well as for applications in enhanced carbon sequestration and renewable energy. Here, we undertook a label free proteomic approach to investigate the effect of extended light (LL) or extended dark (DD) conditions on the unicellular cyanobacterium Crocosphaera subtropica ATCC 51142. We quantified 2287 proteins, of which 603 proteins were significantly different between the two growth conditions. These proteins represent several biological processes, including photosynthetic electron transport, carbon fixation, stress responses, translation, and protein degradation. Results highlight the regulation of proteases including ATP dependent Clp-proteases (endopeptidases) and metalloproteases and may suggest dynamic responses of proteases to extended light or dark exposure to regulate protein turnover or protein quality control mechanisms. The results enhance our understanding of how Crocosphaera subtropica ATCC51142 adjusts its molecular machinery in response to extended light or dark growth conditions.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Crocosphaera Subtropica (ncbitaxon:2546360)

SUBMITTER: Uma Aryal

PROVIDER: MSV000094518 | MassIVE | Thu Apr 11 12:29:00 BST 2024

REPOSITORIES: MassIVE

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Proteomic analysis of unicellular cyanobacterium Crocosphaera subtropica ATCC 51142 under extended light or dark growth

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