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Single-cell proteomics differentiates Arabidopsis root cell types

ABSTRACT: Here we explore the feasibility of single-cell proteomics on plant samples. We focus on Arabidopsis thaliana, examining isolated single cells from the cortex and endodermis, which are two adjacent root cell-types derived from a common stem cell.

INSTRUMENT(S): Q Exactive Plus

ORGANISM(S): Arabidopsis Thaliana (ncbitaxon:3702)

SUBMITTER: Justin Walley

PROVIDER: MSV000094558 | MassIVE | Wed Apr 17 12:27:00 BST 2024

SECONDARY ACCESSION(S): PXD051530

REPOSITORIES: MassIVE

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Single-cell proteomics differentiates Arabidopsis root cell types.

Montes Christian C Zhang Jingyuan J Nolan Trevor M TM Walley Justin W JW

The New phytologist 20240624 5

Single-cell proteomics (SCP) is an emerging approach to resolve cellular heterogeneity within complex tissues of multi-cellular organisms. Here, we demonstrate the feasibility of SCP on plant samples using the model plant Arabidopsis thaliana. Specifically, we focused on examining isolated single cells from the cortex and endodermis, which are two adjacent root cell types derived from a common stem cell lineage. From 756 root cells, we identified 3763 proteins and 1118 proteins/cell. Ultimately, ...[more]

PMID: 38923440

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