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Identification of proteolytic events during C2C12 myoblast differentiation

ABSTRACT: Identification of product of proteolysis during C2C12 myoblast differentiation using subtiligase N-terminomics. Different cell populations collected during a time-course of differentiation (4 days) were used for N-terminal labeling in a forward degradomics approach (n=2). Day0 population= Myoblasts, Day1 populations= live cells and dead cells, Day4 populations= Myotubes and Reserve cells. Additionally, cleavages events generated by mouse caspase-3 at early stages of differentiation (Day 0 and 1) was evaluated using a reverse degradomics approach on myoblasts and live cells (n=2).

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Mus Musculus (ncbitaxon:10090)

SUBMITTER: Olivier Julien

PROVIDER: MSV000094657 | MassIVE | Wed May 01 17:28:00 BST 2024

REPOSITORIES: MassIVE

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An atlas of caspase cleavage events in differentiating muscle cells.

Gomez-Cardona Erik E Dehkordi Mahshid H MH Van Baar Kolden K Vitkauskaite Aiste A Julien Olivier O Fearnhead Howard O HO

Protein science : a publication of the Protein Society 20240901 9

Executioner caspases, such as caspase-3, are known to induce apoptosis, but in other contexts, they can control very different fates, including cell differentiation and neuronal plasticity. While hundreds of caspase substrates are known to be specifically targeted during cell death, we know very little about how caspase activity brings about non-apoptotic fates. Here, we report the first proteome identification of cleavage events in C2C12 cells undergoing myogenic differentiation and its compari ...[more]

PMID: 39180494

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