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Top-down mass spectrometry analysis of capsid proteins of recombinant adeno-associated virus using multiple ion activations and proton transfer charge reduction

ABSTRACT: Here, we present our investigation of the viral proteins of the capsid of adeno-associated viruses via top-down mass spectrometry on the LC timescale. Characterization was improved through the use of proton transfer charge reduction

INSTRUMENT(S): Orbitrap Ascend

ORGANISM(S): Human Aav

SUBMITTER: Luca Fornelli

PROVIDER: MSV000095045 | MassIVE | Fri Jun 14 13:02:00 BST 2024

REPOSITORIES: MassIVE

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Top-down mass spectrometry analysis of capsid proteins of recombinant adeno-associated virus using multiple ion activations and proton transfer charge reduction.

Kline Jake T JT Huang Jingjing J Lieu Linda B LB Srzentić Kristina K Bergen David D Mullen Christopher C McAlister Graeme C GC Durbin Kenneth R KR Melani Rafael D RD Fornelli Luca L

Proteomics 20240905

Adeno-associated viruses (AAVs) are common vectors for emerging gene therapies due to their lack of pathogenicity in humans. Here, we present our investigation of the viral proteins (i.e., VP1, VP2, and VP3) of the capsid of AAVs via top-down mass spectrometry (MS). These proteins, ranging from 59 to 81 kDa, were chromatographically separated using hydrophilic interaction liquid chromatography and characterized in the gas-phase by high-resolution Orbitrap Fourier transform MS. Complementary ion ...[more]

PMID: 39233542

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