Proteomics

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Cell Storage Conditions Impact Single-Cell Proteomic Landscape


ABSTRACT: Three storage conditions were compared in 293T cells: (1) 37C (control), (2) 4C overnight, and (3) -80C storage followed by liquid nitrogen preservation. Either cold storage induced significant alterations in cell diameter, circularity, and proteome composition. This study highlights the importance of preserving cells under viable conditions to maintain the integrity of the proteome and underscore the necessity of optimizing cell storage protocols to ensure accurate representation of the cellular proteome, particularly in the context of SCP studies. By elucidating the intricate interplay between storage conditions, cellular morphology, and protein expression, this study contributes valuable insights towards advancing SCP methodologies and enhancing our understanding of cellular heterogeneity.

INSTRUMENT(S): timsTOF SCP

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Jesse G. Meyer  

PROVIDER: MSV000095162 | MassIVE | Wed Jun 26 22:33:00 BST 2024

REPOSITORIES: MassIVE

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