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The PNUTS phosphatase complex controls transcription pause release

ABSTRACT: Gene expression is regulated by controlling distinct steps of the transcriptional cycle, including initiation, pausing, elongation, and termination. Kinases phosphorylate RNA Polymerase II and associated factors to control transitions between these steps and act as central gene regulatory nodes. Similarly, phosphatases that dephosphorylate these components are emerging as important regulators of transcription, though their roles remain less well understood. Here we discover that the PNUTS-PP1 phosphatase complex plays an essential role in controlling transcription pause release in addition to its previously described function in transcription termination. Transcription pause release by the PNUTS complex is essential for almost all RNA Pol II-dependent gene transcription, relies on its PP1 phosphatase subunit, and controls the phosphorylation of factors required for pause release and elongation. Together, this reveals an essential new role for a phosphatase complex in transcription pause release and shows that the PNUTS complex is essential for RNA Poll II-dependent transcription.

INSTRUMENT(S): Orbitrap Fusion Lumos, Exactive Plus

ORGANISM(S): Mus Musculus (ncbitaxon:10090)

SUBMITTER: Arminja Nadine Kettenbach

PROVIDER: MSV000095177 | MassIVE | Fri Jun 28 09:35:00 BST 2024

SECONDARY ACCESSION(S): PXD053493

REPOSITORIES: MassIVE

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Elongation/termination factor exchange mediated by PP1 phosphatase orchestrates trancription termination

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Control of RNA pol II speed by PNUTS-PP1 and Spt5 dephosphorylation facilitates termination by a “sitting duck torpedo” mechanism

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