Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Unraveling Cecal Alterations in Clostridioides difficile Colonized Mice through a Comprehensive Metabolic Profile of 289 Compound

ABSTRACT: Unraveling Cecal Alterations in Clostridioides difficile Colonized Mice through a Comprehensive Metabolic Profile of 289 Compound

INSTRUMENT(S): Xevo TQD (Waters instrument model), GC-MS/MS EVOQ 456 Bruker

ORGANISM(S): C57bl/6

SUBMITTER: Olga Deda

PROVIDER: MSV000095285 | MassIVE | Wed Jul 10 10:19:00 BST 2024

REPOSITORIES: MassIVE

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Unraveling Cecal Alterations in <i>Clostridioides difficile</i> Colonized Mice through Comprehensive Metabolic Profiling.

Project description:The disruption of gut microbiota caused by antibiotics favors the intestinal colonization of Clostridioides difficile - a Gram-positive, spore-forming anaerobic bacterium that causes potentially fatal gastrointestinal infections. In an endeavor to elucidate the complexities of the gut-brain axis in the context of Clostridium difficile infection (CDI), a murine model has been used to investigate the potential effects of antibiotic administration and subsequent colonization by C. difficile, as well as the impact of three different 10-day treatments (metronidazole, probiotics, and fecal microbiota transplantation), on the cecal metabolome for the first time. This follows our previous research which highlighted the metabolic effect of CDI and these treatments in the brain and employs the same four different metabolomics-based methods (targeted GC-MS/MS, targeted HILIC-MS/MS, untargeted RP-LC-HRMS/MS and untargeted GC-MS). A total of 286 unique metabolites have been identified in the mouse cecal profiles and statistical analysis revealed that CDI, as well as the subsequent treatments, significantly alters cecal metabolites and lipids implicated in various biochemical pathways centered around amino acid metabolism, glycerophospholipid metabolism, and central carbon metabolism. To our knowledge, this study represents the first exploration of the effects of C. difficile-induced colitis and potential treatments on the cecal tissue metabolome.

| S-EPMC11629376 | biostudies-literature

Impact of compound VU0026921 on S. aureus transcriptional profile

Project description:Compound VU0026921 is antimicrobial to S. aureus in a manner modulated by Mn. To elucidate information regarding the mechanism of action of VU0026921, we performed RNA sequencing of S. aureus exposed to VU0026921 in the presence or absence of excess Mn

2020-10-07 | GSE128062 | GEO

Impact of compound VU0026921 on S. aureus transcriptional profile

Project description:Impact of compound VU0026921 on S. aureus transcriptional profile

| PRJNA526226 | ENA

Unraveling potential gene biomarkers for dengue infection through RNA sequencing

Project description:Unraveling potential gene biomarkers for dengue infection through RNA sequencing

| PRJNA1171376 | ENA

Gene expression analysis of leukemia-initiating cells of compound URE-/+::Msh2-/- mice

Project description:Expression profiling of FACS purified Lin-cKit+ cells from compound URE-/+::Msh2-/- mice with AML and control animals

2015-09-07 | GSE65668 | GEO

Compound C prevents the unfolded protein response during glucose deprivation through a mechanism independent of AMPK and BMP signaling.

Project description:Inhibiting the unfolded protein response (UPR) can be a therapeutic approach, especially for targeting the tumor microenvironment. We found that compound C (also known as dorsomorphin) prevented the UPR and exerted enhanced cytotoxicity during glucose deprivation. The UPR-inhibiting activity of compound C was not associated with either AMPK or BMP signaling inhibition. To induce the UPR, we treated HT1080 cells for 18 hours under ER stress conditions by adding 10 mM 2-Deoxy-D-glucose (2DG) to culture medium. UPR inhibitors (compound C, versipelostatin and phenformin) were added just before 2DG was added in medium. Total 8 samples were prepared for RNA extraction and hybridization on Affymetrix microarrays.

2012-10-11 | E-GEOD-32911 | biostudies-arrayexpress

Unraveling cysteine deficiency-associated rapid weight loss

Project description:Unraveling cysteine deficiency-associated rapid weight loss

| PRJNA1176697 | ENA

Gene expression analysis of leukemia-initiating cells of preleukemic compound URE-/+::Msh2-/- mice

Project description:Expression profiling of FACS purified Lin-cKit+ cells from preleukemic compound URE-/+::Msh2-/- mice and control animals (two separate pools of 3 mice each)

2015-09-07 | GSE65669 | GEO

Affinity purification using compound-immobilized beads

Project description:A drug target was explored by affinity purification using the compound-immobilized beads.

2019-07-10 | PXD010349 | JPOST Repository

Compound C prevents the unfolded protein response during glucose deprivation through a mechanism independent of AMPK and BMP signaling.

2012-10-11 | GSE32911 | GEO