Proteomics

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Burger_et_al_ilicicolin_metabolomics

ABSTRACT: In this study, the ilicicolin biosynthetic gene cluster (BGC) in Trichoderma reesei was genetically activated, and a series of gene cluster enzyme deletions were performed. Mycelia were harvested and subjected to polar extraction. The resulting polar extracts were analyzed using an untargeted lipidomics workflow in positive ionization mode (for further methodological details, refer to the publication). The dataset includes: Metabolomics data: 16 raw files (4 experimental conditions, in quadruplicates) measured using trapped ion mobility spectrometry (TIMS). Molecular networking data: 2 raw files (from 2 conditions) measured without TIMS to obtain improved fragmentation spectra. Molecular networking analysis was conducted with MZmine 4, and the corresponding batch file is included as supplementary data.

INSTRUMENT(S): timsTOF Pro

ORGANISM(S): Trichoderma Reesei (ncbitaxon:51453)

SUBMITTER: Matthias Schittmayer  

PROVIDER: MSV000095813 | MassIVE |

REPOSITORIES: MassIVE

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Discovery of the antifungal compound ilicicolin K through genetic activation of the ilicicolin biosynthetic pathway in Trichoderma reesei.

Burger Isabella I   Schmal Matthias M   Peikert Kathrin K   Fourtis Lukas L   Suster Christoph C   Stanetty Christian C   Schnalzer Dominik D   Hufnagel Barbara B   Böttcher Thomas T   Birner-Gruenberger Ruth R   Mach Robert L RL   Mach-Aigner Astrid R AR   Schittmayer Matthias M   Zimmermann Christian C  

Biotechnology for biofuels and bioproducts 20250311 1

<h4>Background</h4>Given the global rise in antimicrobial resistance, the discovery of novel antimicrobial agents and production processes thereof are of utmost importance. To this end we have activated the gene cluster encoding for the biosynthesis of the potent antifungal compound ilicicolin H in the fungus Trichoderma reesei. While the biosynthetic gene cluster (BGC) is silent under standard cultivation conditions, we achieved BGC activation by genetically overexpressing the transcription fac  ...[more]

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