Identification of disulfide bond-linking sites in biosynthesized platelet factor 4 by establishing a partial reduction method without alkylation
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ABSTRACT: In this research, a bacterial expression system was established to efficiently generate recombinant human PF4 (rhPF4), and the accuracy of the sequence was validated by liquid chromatography-high-resolution mass spectrometry. A relatively straightforward alkylation-independent partial reduction approach was developed to identify the two disulfide bond sites in PF4, which are critical for its structural integrity and functionality.
INSTRUMENT(S): LTQ-Orbitrap Velos,UltiMate 3000 Standard (SD) HPLC systems
ORGANISM(S): Homo Sapiens (ncbitaxon:9606)
SUBMITTER: Wenrui Hu
PROVIDER: MSV000096779 | MassIVE | Sun Jan 05 12:11:00 GMT 2025
SECONDARY ACCESSION(S): PXD059440
REPOSITORIES: MassIVE
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