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1. Mitochondrial Protein Identification; 2. Redox Proteomics Identification; 3. Quantitative Cellular Proteomics

ABSTRACT: 1. Quantitative Proteomic Analysis of Isolated Mitochondria. Each group has three replicates: Glc group: Glucose starvation group. GlcFH group: FH activity inhibition combined with glucose starvation group. 2. Quantitative proteomics analysis was performed to assess intracellular protein disulfide bond levels. The experiment included the following groups, each with three replicates:UOK group: UOK262 cells under glucose starvation; UOKFH group: UOK262 cells overexpressing FH under glucose starvation. V786O group: 786-O cells under glucose starvation; XCT786O group: 786-O cells overexpressing XCT under glucose starvation. 3. Quantitative Proteomics Analysis of Cellular Protein Expression Differences, Each group has three replicates: Glcn group: glucose starvation group. Glcp group: glucose sufficiency group

INSTRUMENT(S): 1. Orbitrap melting fluorescence coupled to an EASY nanoLC 1200 system (Thermo Fisher Scientific, MA, USA), 3. Tandem EASY-nanoLC1200 with Orbitrap Fusion Lumos (Thermo Fisher Scientific, MA, USA), 2.Orbitrap Astral mass spectrometer coupled with a Vanquish Neo UHPLC system (Thermo Fisher Scientific)

ORGANISM(S): Human 2. Human 1. Human 3. Human

SUBMITTER: Guowang Xu

PROVIDER: MSV000097130 | MassIVE | Sun Feb 16 08:07:00 GMT 2025

REPOSITORIES: MassIVE

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