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Calcium Handling in Breast Cancer

ABSTRACT: Extracellular calcium export by the breast ductal epithelium is crucial during lactation and plays a significant role in breast cancer progression. Intraductal calcium deposition is a hallmark of aggressive premalignant lesions. This study tested the hypothesis that microbiome-derived extracellular vesicles (EVs) influence calcium modulation within premalignant breast cancer lesions. Based on the analysis of plasma, serum, saliva, and tissue collected from breast cancer patients and controls (N=150), Lactobacillus rhamnosus (Lr) was chosen as the model microbiota. In a BT-474 human breast cancer cell line monolayer culture under acute calcium stress, Lr EVs enhanced intracellular calcium intake. In a BT474 3D spheroid model under chronic calcium stress, Lr EVs increased extracellular calcium deposition and the mRNA expression of calcium export channel calcium pump isoform 2 (PMCA2) and stromal interaction molecule 1 (STIM1) in a dose-dependent manner. We propose that Lr EVs influence calcium regulation and mineral deposition, thereby affecting premalignant breast cancer progression.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Lactobacillus (ncbitaxon:1578) Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Alessandra Luchini

PROVIDER: MSV000097290 | MassIVE | Tue Mar 11 11:35:00 GMT 2025

SECONDARY ACCESSION(S): PXD061715

REPOSITORIES: MassIVE

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