Dataset Information

To study the acetylation of human serum albumin (HSA) in presence and absence of Aldicarb using 2-Naphthyl Acetate (2NA) as substrate and digestion with Pepsin.

ABSTRACT: This is study done for academic purpose and funded by ICMR, New Delhi, India, sanctioned project titled:- To study the acetylation of human serum albumin (HSA) in presence and absence of Aldicarb using 2-Naphthyl Acetate (2NA) as substrate and digestion with Pepsin. Project File No. 5/4/1-25/2020-NCD-1. Sample processing protocol: For this, 1 ml of 1 mg/ml solution of fatty acid free human albumin (SIGMA) in 10 mM Tris HCl buffer, pH 7.38 was incubated with Aldicarb (in Acetonitrile)(working concentration of 5 microMolar (Organocarbamate) for 1 hour at 37 degree Celsius. Further, acetylation was done using 2NA (prepared in Acetonitrile) for 45 minutes at 37 degree Celsius. 100 microlitre of each aliquot was denatured by boiling with 10 mM dithiothreitol for 10 minutes followed by the addition of Iodoacetamide (90 mM, SIGMA) and incubation for 30 mins in the dark at 37 degree Celsius. These were then subjected to Pepsin digestion (2 microgram, Promega, MS grade, prepared in acidic water).Next, samples to be digested by pepsin were incubated for 2 hours at 37 degree Celsius. Pepsin digestion was stopped by heating samples at 95 degree Celsius. The sample clean-up with C18 resins column was performed and the aliquots were then sent for data acquisition. Data Processing protocol: The MaxQuant software (2.0.3.1) was used for the analysis of data. Under group-specific parameters acetyl K, acetyl (N-term), and (acetyl protein N-term) were selected as variable modifications whereas the Carbamidomethyl (C) was considered a fixed modification. The label-free quantification was done and the albumin sequence file (accession: P02768) was taken as a reference for understanding the analysis. For digestion, Pepsin enzyme was added to MaxQuant enzyme file. The albumin sequence file (accession: P02768) was taken as a reference for understanding the analysis. Description of the raw files is as follow. HSA_Aldicarb is Human serum albumin treated with Aldicarb and HSA_Aldicarb_2NA is Human serum Albumin treated with Aldicarb and then substrate (2NA) was added.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Dibyajyoti Banerjee

PROVIDER: MSV000097370 | MassIVE | Wed Mar 19 03:44:00 GMT 2025

REPOSITORIES: MassIVE

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Project description:This is study done for academic purpose and funded by ICMR, New Delhi, India, sanctioned project titled:- To study the acetylation of human serum albumin (HSA) in presence and absence of Chlorpyrifos using 2-Naphthyl Acetate (2NA) as substrate and digestion with Pepsin. Project File No. 5/4/1-25/2020-NCD-1. Sample processing protocol: For this, 1 ml of 1 mg/ml solution of fatty acid free human albumin (SIGMA) in 10 mM Tris HCl buffer, pH 7.38 was incubated with Chlorpyrifos (in Acetonitrile)(working concentration of 5 microMolar for 1 hour at 37 degree Celsius. Further, acetylation was done using 2NA (prepared in Acetonitrile) for 45 minutes at 37 degree Celsius. 100 microlitre of each aliquot was denatured by boiling with 10 mM dithiothreitol for 10 minutes followed by the addition of Iodoacetamide (90 mM, SIGMA) and incubation for 30 mins in the dark at 37 degree Celsius. These were then subjected to Pepsin digestion (2 microgram, Promega, MS grade, prepared in acidic water).Next, samples to be digested by pepsin were incubated for 2 hours at 37 degree Celsius. Pepsin digestion was stopped by heating samples at 95 degree Celsius. The sample clean-up with C18 resins column was performed and the aliquots were then sent for data acquisition. Data Processing protocol: The MaxQuant software (2.0.3.1) was used for the analysis of data. Under group-specific parameters acetyl K, acetyl (N-term), and (acetyl protein N-term) were selected as variable modifications whereas the Carbamidomethyl (C) was considered a fixed modification. The label-free quantification was done and the albumin sequence file (accession: P02768) was taken as a reference for understanding the analysis. For digestion, Pepsin enzyme was added to MaxQuant enzyme file. The albumin sequence file (accession: P02768) was taken as a reference for understanding the analysis. Description of the raw files is as follow. HSA_Chlorpyrifos is Human serum albumin treated with Chlorpyrifos and HSA_Chlorpyrifos _2NA is Human serum Albumin treated with Chlorpyrifos and then substrate (2NA) was added.

Project description:This is study done for academic purpose and funded by ICMR, New Delhi, India, sanctioned project titled:- To study the acetylation of human serum albumin (HSA) in presence and absence of Carbaryl using 2-Naphthyl Acetate (2NA) as substrate and digestion with Pepsin. Project File No. 5/4/1-25/2020-NCD-1. Sample processing protocol: For this, 1 ml of 1 mg/ml solution of fatty acid free human albumin (SIGMA) in 10 mM Tris HCl buffer, pH 7.38 was incubated with Carbaryl (in Acetonitrile)(working concentration of 5 microMolar (Organocarbamate) for 1 hour at 37 degree Celsius. Further, acetylation was done using 2NA (prepared in Acetonitrile) for 45 minutes at 37 degree Celsius. 100 microlitre of each aliquot was denatured by boiling with 10 mM dithiothreitol for 10 minutes followed by the addition of Iodoacetamide (90 mM, SIGMA) and incubation for 30 mins in the dark at 37 degree Celsius. These were then subjected to Pepsin digestion (2 microgram, Promega, MS grade, prepared in acidic water).Next, samples to be digested by pepsin were incubated for 2 hours at 37 degree Celsius. Pepsin digestion was stopped by heating samples at 95 degree Celsius. The sample clean-up with C18 resins column was performed and the aliquots were then sent for data acquisition. Data Processing protocol: The MaxQuant software (2.0.3.1) was used for the analysis of data. Under group-specific parameters acetyl K, acetyl (N-term), and (acetyl protein N-term) were selected as variable modifications whereas the Carbamidomethyl (C) was considered a fixed modification. The label-free quantification was done and the albumin sequence file (accession: P02768) was taken as a reference for understanding the analysis. For digestion, Pepsin enzyme was added to MaxQuant enzyme file. The albumin sequence file (accession: P02768) was taken as a reference for understanding the analysis. Description of the raw files is as follow. HSA_Carbaryl is Human serum albumin treated with Carbaryl and HSA_Carbaryl_2NA is Human serum Albumin treated with Carbaryl and then substrate (2NA) was added.

Project description:This is study done for academic purpose and funded by ICMR, New Delhi, India, sanctioned project titled:- To study the acetylation of human serum albumin (HSA) in presence and absence of Aminocarb using 2-Naphthyl Acetate (2NA) as substrate and digestion with Pepsin. Project File No. 5/4/1-25/2020-NCD-1. Sample processing protocol: For this, 1 ml of 1 mg/ml solution of fatty acid free human albumin (SIGMA) in 10 mM Tris HCl buffer, pH 7.38 was incubated with Aminocarb (in Acetonitrile)(working concentration of 5 microMolar (Organocarbamate) for 1 hour at 37 degree Celsius. Further, acetylation was done using 2NA (prepared in Acetonitrile) for 45 minutes at 37 degree Celsius. 100 microlitre of each aliquot was denatured by boiling with 10 mM dithiothreitol for 10 minutes followed by the addition of Iodoacetamide (90 mM, SIGMA) and incubation for 30 mins in the dark at 37 degree Celsius. These were then subjected to Pepsin digestion (2 microgram, Promega, MS grade, prepared in acidic water).Next, samples to be digested by pepsin were incubated for 2 hours at 37 degree Celsius. Pepsin digestion was stopped by heating samples at 95 degree Celsius. The sample clean-up with C18 resins column was performed and the aliquots were then sent for data acquisition. Data Processing protocol: The MaxQuant software (2.0.3.1) was used for the analysis of data. Under group-specific parameters acetyl K, acetyl (N-term), and (acetyl protein N-term) were selected as variable modifications whereas the Carbamidomethyl (C) was considered a fixed modification. The label-free quantification was done and the albumin sequence file (accession: P02768) was taken as a reference for understanding the analysis. For digestion, Pepsin enzyme was added to MaxQuant enzyme file. The albumin sequence file (accession: P02768) was taken as a reference for understanding the analysis. Description of the raw files is as follow. HSA_Aminocarb is Human serum albumin treated with Aminocarb and HSA_Aminocarb_2NA is Human serum Albumin treated with Aminocarb and then substrate (2NA) was added.

Project description:This is study done for academic purpose and funded by ICMR, New Delhi, India, sanctioned project titled:- To study the acetylation of human serum albumin (HSA) in presence and absence of Rivastigmine using 2-Naphthyl Acetate (2NA) as substrate and digestion with Pepsin. Project File No. 5/4/1-25/2020-NCD-1. Sample processing protocol: For this, 1 ml of 1 mg/ml solution of fatty acid free human albumin (SIGMA) in 10 mM Tris HCl buffer, pH 7.38 was incubated with Rivastigmine (in Acetonitrile)(working concentration of 5 microMolar for 1 hour at 37 degree Celsius. Further, acetylation was done using 2NA (prepared in Acetonitrile) for 45 minutes at 37 degree Celsius. 100 microlitre of each aliquot was denatured by boiling with 10 mM dithiothreitol for 10 minutes followed by the addition of Iodoacetamide (90 mM, SIGMA) and incubation for 30 mins in the dark at 37 degree Celsius. These were then subjected to Pepsin digestion (2 microgram, Promega, MS grade, prepared in acidic water).Next, samples to be digested by pepsin were incubated for 2 hours at 37 degree Celsius. Pepsin digestion was stopped by heating samples at 95 degree Celsius. The sample clean-up with C18 resins column was performed and the aliquots were then sent for data acquisition. Data Processing protocol: The MaxQuant software (2.0.3.1) was used for the analysis of data. Under group-specific parameters acetyl K, acetyl (N-term), and (acetyl protein N-term) were selected as variable modifications whereas the Carbamidomethyl (C) was considered a fixed modification. The label-free quantification was done and the albumin sequence file (accession: P02768) was taken as a reference for understanding the analysis. For digestion, Pepsin enzyme was added to MaxQuant enzyme file. The albumin sequence file (accession: P02768) was taken as a reference for understanding the analysis. Description of the raw files is as follow. HSA_Rivastigmine is Human serum albumin treated with Rivastigmine and HSA_Rivastigmine _2NA is Human serum Albumin treated with Rivastigmine and then substrate (2NA) was added.

Project description:This is study done for academic purpose and funded by ICMR, New Delhi, India, sanctioned project titled:- To study the acetylation of human serum albumin (HSA) in presence and absence of Chlorpyrifos_oxon using 2-Naphthyl Acetate (2NA) as substrate and digestion with Pepsin. Project File No. 5/4/1-25/2020-NCD-1. Sample processing protocol: For this, 1 ml of 1 mg/ml solution of fatty acid free human albumin (SIGMA) in 10 mM Tris HCl buffer, pH 7.38 was incubated with Chlorpyrifos_oxon (in Acetonitrile)(working concentration of 5 microMolar (Organophosphorous) for 1 hour at 37 degree Celsius. Further, acetylation was done using 2NA (prepared in Acetonitrile) for 45 minutes at 37 degree Celsius. 100 microlitre of each aliquot was denatured by boiling with 10 mM dithiothreitol for 10 minutes followed by the addition of Iodoacetamide (90 mM, SIGMA) and incubation for 30 mins in the dark at 37 degree Celsius. These were then subjected to Pepsin digestion (2 microgram, Promega, MS grade, prepared in acidic water).Next, samples to be digested by pepsin were incubated for 2 hours at 37 degree Celsius. Pepsin digestion was stopped by heating samples at 95 degree Celsius. The sample clean-up with C18 resins column was performed and the aliquots were then sent for data acquisition. Data Processing protocol: The MaxQuant software (2.0.3.1) was used for the analysis of data. Under group-specific parameters acetyl K, acetyl (N-term), and (acetyl protein N-term) were selected as variable modifications whereas the Carbamidomethyl (C) was considered a fixed modification. The label-free quantification was done and the albumin sequence file (accession: P02768) was taken as a reference for understanding the analysis. For digestion, Pepsin enzyme was added to MaxQuant enzyme file. The albumin sequence file (accession: P02768) was taken as a reference for understanding the analysis. Description of the raw files is as follow. HSA_Chlorpyrifos_oxon is Human serum albumin treated with Chlorpyrifos_oxon and HSA_Chlorpyrifos_oxon_2NA is Human serum Albumin treated with Chlorpyrifos_oxon and then substrate (2NA) was added.

Project description:This is study done for academic purpose and funded by ICMR, New Delhi, India, sanctioned project titled:- To study the acetylation of human serum albumin (HSA) in presence and absence of Malaoxon using 2-Naphthyl Acetate (2NA) as substrate and digestion with Pepsin. Project File No. 5/4/1-25/2020-NCD-1. Sample processing protocol: For this, 1 ml of 1 mg/ml solution of fatty acid free human albumin (SIGMA) in 10 mM Tris HCl buffer, pH 7.38 was incubated with Malaoxon (in Acetonitrile)(working concentration of 5 microMolar for 1 hour at 37 degree Celsius. Further, acetylation was done using 2NA (prepared in Acetonitrile) for 45 minutes at 37 degree Celsius. 100 microlitre of each aliquot was denatured by boiling with 10 mM dithiothreitol for 10 minutes followed by the addition of Iodoacetamide (90 mM, SIGMA) and incubation for 30 mins in the dark at 37 degree Celsius. These were then subjected to Pepsin digestion (2 microgram, Promega, MS grade, prepared in acidic water).Next, samples to be digested by pepsin were incubated for 2 hours at 37 degree Celsius. Pepsin digestion was stopped by heating samples at 95 degree Celsius. The sample clean-up with C18 resins column was performed and the aliquots were then sent for data acquisition. Data Processing protocol: The MaxQuant software (2.0.3.1) was used for the analysis of data. Under group-specific parameters acetyl K, acetyl (N-term), and (acetyl protein N-term) were selected as variable modifications whereas the Carbamidomethyl (C) was considered a fixed modification. The label-free quantification was done and the albumin sequence file (accession: P02768) was taken as a reference for understanding the analysis. For digestion, Pepsin enzyme was added to MaxQuant enzyme file. The albumin sequence file (accession: P02768) was taken as a reference for understanding the analysis. Description of the raw files is as follow. HSA_Malaoxon is Human serum albumin treated with Malaoxon and HSA_Malaoxon _2NA is Human serum Albumin treated with Malaoxon and then substrate (2NA) was added.

Project description:This is study done for academic purpose and funded by ICMR, New Delhi, India, sanctioned project titled:- To study the acetylation of human serum albumin (HSA) in presence and absence of Pyridostigmine_Bromide using 2-Naphthyl Acetate (2NA) as substrate and digestion with Pepsin. Project File No. 5/4/1-25/2020-NCD-1. Sample processing protocol: For this, 1 ml of 1 mg/ml solution of fatty acid free human albumin (SIGMA) in 10 mM Tris HCl buffer, pH 7.38 was incubated with Pyridostigmine_Bromide (in Acetonitrile)(working concentration of 5 microMolar for 1 hour at 37 degree Celsius. Further, acetylation was done using 2NA (prepared in Acetonitrile) for 45 minutes at 37 degree Celsius. 100 microlitre of each aliquot was denatured by boiling with 10 mM dithiothreitol for 10 minutes followed by the addition of Iodoacetamide (90 mM, SIGMA) and incubation for 30 mins in the dark at 37 degree Celsius. These were then subjected to Pepsin digestion (2 microgram, Promega, MS grade, prepared in acidic water).Next, samples to be digested by pepsin were incubated for 2 hours at 37 degree Celsius. Pepsin digestion was stopped by heating samples at 95 degree Celsius. The sample clean-up with C18 resins column was performed and the aliquots were then sent for data acquisition. Data Processing protocol: The MaxQuant software (2.0.3.1) was used for the analysis of data. Under group-specific parameters acetyl K, acetyl (N-term), and (acetyl protein N-term) were selected as variable modifications whereas the Carbamidomethyl (C) was considered a fixed modification. The label-free quantification was done and the albumin sequence file (accession: P02768) was taken as a reference for understanding the analysis. For digestion, Pepsin enzyme was added to MaxQuant enzyme file. The albumin sequence file (accession: P02768) was taken as a reference for understanding the analysis. Description of the raw files is as follow. HSA_ Pyridostigmine_Bromide is Human serum albumin treated with Pyridostigmine_Bromide and HSA_ Pyridostigmine_Bromide _2NA is Human serum Albumin treated with Pyridostigmine_Bromide and then substrate (2NA) was added.

Project description:This is study done for academic purpose and funded by ICMR, New Delhi, India, sanctioned project titled:- To study the acetylation of human serum albumin (HSA) in presence and absence of Neostigmine using 2-Naphthyl Acetate (2NA) as substrate and digestion with Pepsin. Project File No. 5/4/1-25/2020-NCD-1. Sample processing protocol: For this, 1 ml of 1 mg/ml solution of fatty acid free human albumin (SIGMA) in 10 mM Tris HCl buffer, pH 7.38 was incubated with Neostigmine (in Acetonitrile)(working concentration of 5 microMolar for 1 hour at 37 degree Celsius. Further, acetylation was done using 2NA (prepared in Acetonitrile) for 45 minutes at 37 degree Celsius. 100 microlitre of each aliquot was denatured by boiling with 10 mM dithiothreitol for 10 minutes followed by the addition of Iodoacetamide (90 mM, SIGMA) and incubation for 30 mins in the dark at 37 degree Celsius. These were then subjected to Pepsin digestion (2 microgram, Promega, MS grade, prepared in acidic water).Next, samples to be digested by pepsin were incubated for 2 hours at 37 degree Celsius. Pepsin digestion was stopped by heating samples at 95 degree Celsius. The sample clean-up with C18 resins column was performed and the aliquots were then sent for data acquisition. Data Processing protocol: The MaxQuant software (2.0.3.1) was used for the analysis of data. Under group-specific parameters acetyl K, acetyl (N-term), and (acetyl protein N-term) were selected as variable modifications whereas the Carbamidomethyl (C) was considered a fixed modification. The label-free quantification was done and the albumin sequence file (accession: P02768) was taken as a reference for understanding the analysis. For digestion, Pepsin enzyme was added to MaxQuant enzyme file. The albumin sequence file (accession: P02768) was taken as a reference for understanding the analysis. Description of the raw files is as follow. HSA_NMS is Human serum albumin treated with Neostigmine and HSA_NMS _2NA is Human serum Albumin treated with Neostigmine and then substrate (2NA) was added.

Project description:This is study done for academic purpose and funded by ICMR, New Delhi, India, sanctioned project titled:- To study the acetylation of human serum albumin (HSA) in presence and absence of Glyphosate using 2-Naphthyl Acetate (2NA) as substrate and digestion with Pepsin. Project File No. 5/4/1-25/2020-NCD-1. Sample processing protocol: For this, 1 ml of 1 mg/ml solution of fatty acid free human albumin (SIGMA) in 10 mM Tris HCl buffer, pH 7.38 was incubated with Glyphosate (in Acetonitrile)(working concentration of 5 microMolar (Organophosphorous) for 1 hour at 37 degree Celsius. Further, acetylation was done using 2NA (prepared in Acetonitrile) for 45 minutes at 37 degree Celsius. 100 microlitre of each aliquot was denatured by boiling with 10 mM dithiothreitol for 10 minutes followed by the addition of Iodoacetamide (90 mM, SIGMA) and incubation for 30 mins in the dark at 37 degree Celsius. These were then subjected to Pepsin digestion (2 microgram, Promega, MS grade, prepared in acidic water).Next, samples to be digested by pepsin were incubated for 2 hours at 37 degree Celsius. Pepsin digestion was stopped by heating samples at 95 degree Celsius. The sample clean-up with C18 resins column was performed and the aliquots were then sent for data acquisition. Data Processing protocol: The MaxQuant software (2.0.3.1) was used for the analysis of data. Under group-specific parameters acetyl K, acetyl (N-term), and (acetyl protein N-term) were selected as variable modifications whereas the Carbamidomethyl (C) was considered a fixed modification. The label-free quantification was done and the albumin sequence file (accession: P02768) was taken as a reference for understanding the analysis. For digestion, Pepsin enzyme was added to MaxQuant enzyme file. The albumin sequence file (accession: P02768) was taken as a reference for understanding the analysis. Description of the raw files is as follow. HSA_Glyphosate is Human serum albumin treated with Glyphosate and HSA_Glyphosate _2NA is Human serum Albumin treated with Glyphosate and then substrate (2NA) was added.

Project description:This is study done for academic purpose and funded by ICMR, New Delhi, India, sanctioned project titled:- To study the acetylation of human serum albumin (HSA) in presence and absence of Dichlorvos using 2-Naphthyl Acetate (2NA) as substrate and digestion with Pepsin. Project File No. 5/4/1-25/2020-NCD-1. Sample processing protocol: For this, 1 ml of 1 mg/ml solution of fatty acid free human albumin (SIGMA) in 10 mM Tris HCl buffer, pH 7.38 was incubated with Dichlorvos (in Acetonitrile)(working concentration of 5 microMolar (Organophosphorous) for 1 hour at 37 degree Celsius. Further, acetylation was done using 2NA (prepared in Acetonitrile) for 45 minutes at 37 degree Celsius. 100 microlitre of each aliquot was denatured by boiling with 10 mM dithiothreitol for 10 minutes followed by the addition of Iodoacetamide (90 mM, SIGMA) and incubation for 30 mins in the dark at 37 degree Celsius. These were then subjected to Pepsin digestion (2 microgram, Promega, MS grade, prepared in acidic water).Next, samples to be digested by pepsin were incubated for 2 hours at 37 degree Celsius. Pepsin digestion was stopped by heating samples at 95 degree Celsius. The sample clean-up with C18 resins column was performed and the aliquots were then sent for data acquisition. Data Processing protocol: The MaxQuant software (2.0.3.1) was used for the analysis of data. Under group-specific parameters acetyl K, acetyl (N-term), and (acetyl protein N-term) were selected as variable modifications whereas the Carbamidomethyl (C) was considered a fixed modification. The label-free quantification was done and the albumin sequence file (accession: P02768) was taken as a reference for understanding the analysis. For digestion, Pepsin enzyme was added to MaxQuant enzyme file. The albumin sequence file (accession: P02768) was taken as a reference for understanding the analysis. Description of the raw files is as follow. HSA_Dichlorvos is Human serum albumin treated with Dichlorvos and HSA_Dichlorvos _2NA is Human serum Albumin treated with Dichlorvos and then substrate (2NA) was added.

Dataset Information

To study the acetylation of human serum albumin (HSA) in presence and absence of Aldicarb using 2-Naphthyl Acetate (2NA) as substrate and digestion with Pepsin.

Dataset's files

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