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Development and validation of a liquid chromatography-electrospray ionization-time-of-flight mass spectrometry method for induced changes in Nicotiana attenuata leaves during simulated herbivory

ABSTRACT: Metabolite profiling of herbivory-induced changes in Nicotiana attenuata was used to construct a correlation-weighted network to visualize intra- and inter-compound spectra relationships from individual mass features, and to annotate 12 major network hubs, including 4 O-acyl sugars and 6 17-hydroxygeranyllinalool diterpene glycosides, metabolites with recently uncovered anti-herbivore functions.

INSTRUMENT(S): micrOTOF II ESI TOF (Bruker)

SUBMITTER: Steffen Neumann

PROVIDER: MTBLS10 | MetaboLights | 2012-08-14

REPOSITORIES: MetaboLights

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Development and validation of a liquid chromatography-electrospray ionization-time-of-flight mass spectrometry method for induced changes in Nicotiana attenuata leaves during simulated herbivory.

Gaquerel Emmanuel E Heiling Sven S Schoettner Matthias M Zurek Gabriela G Baldwin Ian T IT

Journal of agricultural and food chemistry 20100901 17

A liquid chromatography-electrospray ionization-time-of-flight mass spectrometry (HPLC/ESI-TOF-MS) procedure was developed to characterize changes induced in Nicotiana attenuata leaves 1 h and 5 days after wounding and application of Manduca sexta elicitors. The constancy of the measurement conditions was first confirmed for 22 selected analytes spanning the entire chromatogram. Using the Profile Analysis software, we extracted 367 buckets, which were analyzed by principal component analysis and ...[more]

PMID: 20701244

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