Dataset Information

GC-MS based targeted metabolic profiling identifies changes in the wheat metabolome following deoxynivalenol treatment

ABSTRACT: Fusarium graminearum and related species commonly infest grains causing the devastating plant disease Fusarium head blight (FHB) and the formation of trichothecene mycotoxins. The most relevant toxin is deoxynivalenol (DON), which acts as a virulence factor of the pathogen. FHB is difficult to control and resistance to this disease is a polygenic trait, mainly mediated by the quantitative trait loci (QTL) Fhb1 and Qfhs.ifa-5A. In this study we established a targeted GC-MS based metabolomics workflow comprising a standardized experimental setup for growth, treatment and sampling of wheat ears and subsequent GC-MS analysis followed by data processing and evaluation of QC measures using tailored statistical and bioinformatics tools. This workflow was applied to wheat samples of six genotypes with varying levels of Fusarium resistance, treated with either DON or water, and harvested 0, 12, 24, 48 and 96 hours after treatment. The results suggest that the primary carbohydrate metabolism and transport, the citric acid cycle and the primary nitrogen metabolism of wheat are clearly affected by DON treatment. Most importantly significantly elevated levels of amino acids and derived amines were observed. In particular, the concentrations of the three aromatic amino acids phenylalanine, tyrosine, and tryptophan increased. No clear QTL specific difference in the response could be observed except a generally faster increase in shikimate pathway intermediates in genotypes containing Fhb1. The overall workflow proved to be feasible and facilitated to obtain a more comprehensive picture on the effect of DON on the central metabolism of wheat.

INSTRUMENT(S): 5975C Series GC/MSD (Agilent)

SUBMITTER: Benedikt Warth

PROVIDER: MTBLS112 | MetaboLights | 2014-10-17

REPOSITORIES: MetaboLights

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Dataset's files

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			Action	DRS
	MTBLS112	Other
	FILES	Other
	a_MTBLS112_wheat_metabolome_metabolite_profiling_mass_spectrometry.txt	Txt
	i_Investigation.txt	Txt
	m_MTBLS112_wheat_metabolome_metabolite_profiling_mass_spectrometry_v2_maf.tsv	Tabular

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Publications

GC-MS based targeted metabolic profiling identifies changes in the wheat metabolome following deoxynivalenol treatment.

Warth Benedikt B Parich Alexandra A Bueschl Christoph C Schoefbeck Denise D Neumann Nora Katharina Nicole NK Kluger Bernhard B Schuster Katharina K Krska Rudolf R Adam Gerhard G Lemmens Marc M Schuhmacher Rainer R

Metabolomics : Official journal of the Metabolomic Society 20140927 3

Fusariumgraminearum and related species commonly infest grains causing the devastating plant disease Fusarium head blight (FHB) and the formation of trichothecene mycotoxins. The most relevant toxin is deoxynivalenol (DON), which acts as a virulence factor of the pathogen. FHB is difficult to control and resistance to this disease is a polygenic trait, mainly mediated by the quantitative trait loci (QTL) Fhb1 and Qfhs.ifa-5A. In this study we established a targ ...[more]

PMID: 25972772

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