Dataset Information

Gastropods and Insects Prefer Different Solanum dulcamara Chemotypes

ABSTRACT: Solanum dulcamara (Bittersweet nightshade) shows significant intraspecific variation in glycoalkaloid (GA) composition and concentration. We previously showed that constitutive differences in overall GA levels are correlated with feeding preference of the grey field slug (GFS; Deroceras reticulatum). One particularly preferred accession, ZD11, contained low GA levels, but high levels of previously unknown structurally related uronic acid conjugated compounds (UACs). Here we test whether different slug species as well as insect herbivores show similar feeding preferences among six S. dulcamara accessions with different GA chemotypes. In addition, we investigate whether slug feeding can lead to induced changes in the chemical composition and affect later arriving herbivores. A leaf disc assay using greenhouse-grown plants showed that three slug species similarly preferred accessions with low GA levels. Untargeted metabolomic analyses showed that previous slug feeding consistently increased the levels of N-caffeoyl-putrescine and a structurally related metabolite, but not the levels of GAs and UACs. Slug-induced responses only affected slug preference in one accession. A common garden experiment using the same six accessions revealed that ZD11 received the highest natural gastropod feeding damage, but suffered the lowest damage by specialist flea beetles. The latter preferred to feed on accessions with high GA levels. Our study indicates that different selection pressures imposed by generalist gastropods and specialist insects may explain part of the observed chemical diversity in S. dulcamara.

INSTRUMENT(S): maXis (Bruker)

SUBMITTER: Onno W. Calf

PROVIDER: MTBLS1132 | MetaboLights | 2020-05-16

REPOSITORIES: MetaboLights

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Dataset's files

Source:

			Action	DRS
	MTBLS1132	Other
	FILES	Other
	a_MTBLS1132_20190624_sl12_metabolite_profiling_mass_spectrometry.txt	Txt
	i_Investigation.txt	Txt
	m_MTBLS1132_20190624_sl12_metabolite_profiling_mass_spectrometry_v2_maf.tsv	Tabular

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Publications

Gastropods and Insects Prefer Different Solanum dulcamara Chemotypes.

Calf Onno W OW Huber Heidrun H Peters Janny L JL Weinhold Alexander A Poeschl Yvonne Y van Dam Nicole M NM

Journal of chemical ecology 20180702 2

Solanum dulcamara (Bittersweet nightshade) shows significant intraspecific variation in glycoalkaloid (GA) composition and concentration. We previously showed that constitutive differences in overall GA levels are correlated with feeding preference of the grey field slug (GFS; Deroceras reticulatum). One particularly preferred accession, ZD11, contained low GA levels, but high levels of previously unknown structurally related uronic acid conjugated compounds (UACs). Here we test whether differen ...[more]

PMID: 29961916

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