Dataset Information

Effect of NAC154 transcription factor overexpression on the field grown poplar stem wood metabolome

ABSTRACT: Several xylem-associated regulatory genes have been identified that control processes associated with wood formation in poplar. Prominent among these are the NAC domain transcription factors (NACs). We evaluated the putative involvement of Populus NAC154, a co-ortholog of the Arabidopsis gene SND2, as a regulator of secondary biosynthetic processes in stem internode tissues by interrogating methanol:water extracts from control and transgenic trees. Comprehensive untargeted metabolite profiling was accomplished with a liquid chromatography-mass spectrometry platform that utilized two different chromatographic supports (HILIC and reversed phase) and both positive and negative ionization modes. Evaluation of current and previous year tissues provided datasets for assessing the effects of NAC154 overexpression in wood maturation processes. Phenolic glycoside levels as well as those of oligolignols, sucrose and arginine were modulated with phenotypic and chemotypic traits exhibiting similar trends. Specifically, increased levels of arginine in the NAC154 overexpressing tissues supports a role for the transcription factor in senescence/dormancy-associated processes.

INSTRUMENT(S): Synapt G2-S pos, Synapt G2-S neg

SUBMITTER: Richard Helm

PROVIDER: MTBLS119 | MetaboLights | 2015-01-31

REPOSITORIES: MetaboLights

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Dataset's files

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			Action	DRS
	MTBLS119	Other
	FILES	Other
	a_MTBLS119_nac154oe_metabolite_profiling_mass_spectrometry-1.txt	Txt
	a_MTBLS119_nac154oe_metabolite_profiling_mass_spectrometry-2.txt	Txt
	a_MTBLS119_nac154oe_metabolite_profiling_mass_spectrometry-3.txt	Txt

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Publications

A metabolomic assessment of NAC154 transcription factor overexpression in field grown poplar stem wood.

Jervis Judith J Hildreth Sherry B SB Sheng Xiaoyan X Beers Eric P EP Brunner Amy M AM Helm Richard F RF

Phytochemistry 20150311

Several xylem-associated regulatory genes have been identified that control processes associated with wood formation in poplar. Prominent among these are the NAC domain transcription factors (NACs). Here, the putative involvement of Populus NAC154, a co-ortholog of the Arabidopsis gene SND2, was evaluated as a regulator of "secondary" biosynthetic processes in stem internode tissues by interrogating aqueous methanolic extracts from control and transgenic trees. Comprehensive untargeted metabolit ...[more]

PMID: 25771508

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Project description:Wood, or secondary xylem, is the product of xylogenesis, a developmental process that begins with the proliferation of cambial derivatives and ends with mature xylem fibers and vessels with lignified secondary cell walls. Fully mature xylem has undergone a series of cellular processes, including cell division, cell expansion, secondary wall formation, lignification and programmed cell death. A complex network of interactions between transcriptional regulators and signal transduction pathways controls wood formation. However, the role of metabolites during this developmental process has not been comprehensively characterized. To evaluate the role of metabolites during wood formation, we performed a high spatial resolution metabolomics study of the wood-forming zone of Populus tremula, including laser dissected aspen ray and fiber cells. We show that metabolites show specific patterns within the wood-forming zone, following the differentiation process from cell division to cell death. The data from profiled laser dissected aspen ray and fiber cells suggests that these two cell types host distinctly different metabolic processes. Furthermore, by integrating previously published transcriptomic and proteomic profiles generated from the same trees, we provide an integrative picture of molecular processes, for example, deamination of phenylalanine during lignification is of critical importance for nitrogen metabolism during wood formation. GC-MS assay of Wood ring and extraxylary tissue is reported in the current study MTBLS1796.UPLC-MS assay of Wood ring and extraxylary tissue is reported in <a href='https://www.ebi.ac.uk/metabolights/MTBLS1797' rel='noopener noreferrer' target='_blank'>MTBLS1797</a>.UPLC-MS assay of Wood fibre and ray cell tissue is reported in <a href='https://www.ebi.ac.uk/metabolights/MTBLS1831' rel='noopener noreferrer' target='_blank'>MTBLS1831</a>.

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