Metabolomics

Dataset Information

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Metabolomic and Metataxonomic Fingerprinting of Human Milk Suggests Compositional Stability over a Natural Term of Breastfeeding to 24 Months


ABSTRACT: Sparse data exist regarding the normal range of composition of maternal milk beyond the first postnatal weeks. This single timepoint, observational study in collaboration with the 'Parenting Science Gang' citizen science group evaluated the metabolite and bacterial composition of human milk from 62 participants (infants aged 3-48 months), nearly 3 years longer than previous studies. We utilised rapid evaporative ionisation mass spectrometry (REIMS) for metabolic fingerprinting and 16S rRNA gene metataxonomics for microbiome composition analysis. Milk expression volumes were significantly lower beyond 24 months of lactation, but there were no corresponding changes in bacterial load, composition, or whole-scale metabolomic fingerprint. Some individual metabolite features (~14%) showed altered abundances in nursling age groups above 24 months. Neither milk expression method nor nursling sex affected metabolite and metataxonomic fingerprints. Self-reported lifestyle factors, including diet and physical traits, had minimal impact on metabolite and metataxonomic fingerprints. Our findings suggest remarkable consistency in human milk composition over natural-term lactation. The results add to previous studies suggesting that milk donation can continue up to 24 months postnatally. Future longitudinal studies will confirm the inter-individual and temporal nature of compositional variations and the use of donor milk as a personalised therapeutic.

INSTRUMENT(S): Direct infusion MS - positive, Direct infusion MS -

SUBMITTER: Simon Cameron 

PROVIDER: MTBLS1370 | MetaboLights | 2022-02-11

REPOSITORIES: MetaboLights

Dataset's files

Source:
Action DRS
MTBLS1370 Other
FILES Other
a_MTBLS1370_NEG_DI-MS_metabolite_profiling.txt Txt
a_MTBLS1370_POS_DI-MS_metabolite_profiling.txt Txt
i_Investigation.txt Txt
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Publications

Metabolomic and Metataxonomic Fingerprinting of Human Milk Suggests Compositional Stability over a Natural Term of Breastfeeding to 24 Months.

Shenker Natalie S NS   Perdones-Montero Alvaro A   Burke Adam A   Stickland Sarah S   McDonald Julie A K JAK   Alexander-Hardiman Kate K   Flanagan James J   Takats Zoltan Z   Cameron Simon J S SJS  

Nutrients 20201111 11


Sparse data exist regarding the normal range of composition of maternal milk beyond the first postnatal weeks. This single timepoint, observational study in collaboration with the 'Parenting Science Gang' citizen science group evaluated the metabolite and bacterial composition of human milk from 62 participants (infants aged 3-48 months), nearly 3 years longer than previous studies. We utilised rapid evaporative ionisation mass spectrometry (REIMS) for metabolic fingerprinting and 16S rRNA gene  ...[more]

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