Metabolomics,Multiomics

Dataset Information

0

Endoplasmic reticulum-derived bodies enable a single-cell chemical defense in Brassicaceae plants


ABSTRACT: Brassicaceae plants have a dual-cell type of chemical defense against herbivory. Here we show a novel single-cell defense involving endoplasmic reticulum (ER)-derived organelles (ER bodies) and the vacuoles. We identify various glucosinolates as endogenous substrates of the ER-body β-glucosidases BGLU23 and BGLU21. Woodlice strongly prefer to eat seedlings of bglu23 bglu21 or a glucosinolate-deficient mutant over wild-type seedlings, confirming that the β-glucosidases have a role in chemical defense: production of toxic compounds upon organellar damage. Deficiency of the Brassicaceae-specific protein NAI2 prevents ER-body formation, which results in a loss of BGLU23 and a loss of resistance to woodlice. Hence, NAI2 that interacts with BGLU23 is essential for sequestering BGLU23 in ER bodies and preventing its degradation. Artificial expression of NAI2 and BGLU23 in non-Brassicaceae plants results in the formation of ER bodies, indicating that acquisition of NAI2 by Brassicaceae plants is a key step in developing their single-cell defense system.

OTHER RELATED OMICS DATASETS IN: PXD016606

INSTRUMENT(S): Liquid Chromatography MS - negative - reverse phase

SUBMITTER: Kenji Yamada 

PROVIDER: MTBLS1383 | MetaboLights | 2020-02-04

REPOSITORIES: MetaboLights

Dataset's files

Source:
Action DRS
MTBLS1383 Other
FILES Other
a_MTBLS1383_LC-MS_negative_reverse-phase_metabolite_profiling.txt Txt
i_Investigation.txt Txt
m_MTBLS1383_LC-MS_negative_reverse-phase_metabolite_profiling_v2_maf.tsv Tabular
Items per page:
1 - 5 of 6
altmetric image

Publications

Endoplasmic reticulum-derived bodies enable a single-cell chemical defense in Brassicaceae plants.

Yamada Kenji K   Goto-Yamada Shino S   Nakazaki Akiko A   Kunieda Tadashi T   Kuwata Keiko K   Nagano Atsushi J AJ   Nishimura Mikio M   Hara-Nishimura Ikuko I  

Communications biology 20200114 1


Brassicaceae plants have a dual-cell type of chemical defense against herbivory. Here, we show a novel single-cell defense involving endoplasmic reticulum (ER)-derived organelles (ER bodies) and the vacuoles. We identify various glucosinolates as endogenous substrates of the ER-body β-glucosidases BGLU23 and BGLU21. Woodlice strongly prefer to eat seedlings of bglu23 bglu21 or a glucosinolate-deficient mutant over wild-type seedlings, confirming that the β-glucosidases have a role in chemical de  ...[more]

Similar Datasets

2020-07-16 | MSV000085767 | GNPS
2020-01-17 | PXD016606 | Pride
2008-06-14 | E-GEOD-6446 | biostudies-arrayexpress
2023-04-14 | GSE98162 | GEO
2023-04-14 | GSE59914 | GEO
2008-08-15 | GSE12300 | GEO
2024-06-30 | GSE250072 | GEO
2014-06-24 | E-GEOD-54875 | biostudies-arrayexpress
2007-06-04 | GSE6446 | GEO
| PRJNA1098072 | ENA