Metabolomics

Dataset Information

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Untargeted metabolomics links glutathione to bacterial cell cycle progression


ABSTRACT: Cell cycle progression requires the coordination of cell growth, chromosome replication, and division. Consequently, a functional cell cycle must be coupled with metabolism. However, direct measurements of metabolome dynamics remained scarce, in particular in bacteria. Here, we describe an untargeted metabolomics approach with synchronized Caulobacter crescentus cells to monitor the relative abundance changes of ~400 putative metabolites as a function of the cell cycle. While the majority of metabolite pools remains homeostatic, ~14% respond to cell cycle progression. In particular, sulfur metabolism is redirected during the G1-S transition, and glutathione levels periodically change over the cell cycle with a peak in late S phase. A lack of glutathione perturbs cell size by uncoupling cell growth and division through dysregulation of KefB, a K+/H+ antiporter. Overall, we here describe the impact of the C. crescentus cell cycle progression on metabolism, and in turn relate glutathione and potassium homeostasis to timely cell division.

INSTRUMENT(S): Liquid Chromatography MS -

SUBMITTER: Johannes Hartl 

PROVIDER: MTBLS1402 | MetaboLights | 2020-10-15

REPOSITORIES: MetaboLights

Dataset's files

Source:
Action DRS
MTBLS1402 Other
FILES Other
a_MTBLS1402_microbore_LC-MS___metabolite_profiling.txt Txt
a_MTBLS1402_nano_LC-MS___metabolite_profiling.txt Txt
i_Investigation.txt Txt
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Publications

Untargeted metabolomics links glutathione to bacterial cell cycle progression.

Hartl Johannes J   Kiefer Patrick P   Kaczmarczyk Andreas A   Mittelviefhaus Maximilian M   Meyer Fabian F   Vonderach Thomas T   Hattendorf Bodo B   Jenal Urs U   Vorholt Julia A JA  

Nature metabolism 20200203 2


Cell cycle progression requires the coordination of cell growth, chromosome replication, and division. Consequently, a functional cell cycle must be coupled with metabolism. However, direct measurements of metabolome dynamics remained scarce, in particular in bacteria. Here, we describe an untargeted metabolomics approach with synchronized <i>Caulobacter crescentus</i> cells to monitor the relative abundance changes of ~400 putative metabolites as a function of the cell cycle. While the majority  ...[more]

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