Dataset Information

Evidence for quorum sensing and differential metabolite production by Ruegeria pomeroyi in response to DMSP

ABSTRACT: Microbial alteration and remineralization of organic matter in the ocean play essential roles in the carbon cycle. The impact of individual compounds as either growth substrates or infochemicals is still poorly constrained in models, particularly for organisms with relatively versatile genomes. Here, we use metabolomics techniques to characterize the response of a heterotrophic marine bacterium, Ruegeria pomeroyi DSS-3, to growth on the abundant algal metabolite dimethylsulfoniopropionate (DMSP). DMSP is a valuable source of reduced carbon and sulfur as well as a chemo-attractant. It is present at higher concentrations during phytoplankton blooms. When cultivated on DMSP, R. pomeroyi synthesized a quorum sensing molecule, N-(3-oxotetradecanoyl)-L-homoserine lactone, which was not synthesized at the same levels during growth on propionate, another carbon substrate. More broadly, we observed differential production of intra- and extracellular metabolites with implications for nitrogen assimilation and microbial cross-feeding. This multi-faceted response suggests that R. pomeroyi uses a two-component regulatory system dependent on the presence of DMSP and high cell densities to activate a suite of metabolic changes that may shape how it accesses nutrients and impacts the surrounding microbial consortium.

OTHER RELATED OMICS DATASETS IN: PRJNA125229

INSTRUMENT(S): TSQ Vantage (Thermo Scientific), Thermo Scientific LTQ FT Ultra

SUBMITTER: Krista Longnecker

PROVIDER: MTBLS157 | MetaboLights | 2015-01-29

REPOSITORIES: MetaboLights

ACCESS DATA

Dataset's files

Source:

			Action	DRS
	MTBLS157	Other
	FILES	Other
	a_MTBLS157_NEG2_ENDO_metabolite_profiling_mass_spectrometry.txt	Txt
	a_MTBLS157_NEG2_EXO_metabolite_profiling_mass_spectrometry.txt	Txt
	a_MTBLS157_NEG_ENDO_metabolite_profiling_mass_spectrometry.txt	Txt

Items per page:

1 - 5 of 19

Publications

Evidence for quorum sensing and differential metabolite production by a marine bacterium in response to DMSP.

Johnson Winifred M WM Kido Soule Melissa C MC Kujawinski Elizabeth B EB

The ISME journal 20160216 9

Microbes, the foundation of the marine foodweb, do not function in isolation, but rather rely on molecular level interactions among species to thrive. Although certain types of interactions between autotrophic and heterotrophic microorganisms have been well documented, the role of specific organic molecules in regulating inter-species relationships and supporting growth are only beginning to be understood. Here, we examine one such interaction by characterizing the metabolic response of a hetero ...[more]

PMID: 26882264

Similar Datasets

Project description:To effectively monitor microbial populations in acidic environments and bioleaching systems, a comprehensive 50-mer-based oligonucleotide microarray was developed based on most of the known genes associated with the acidophiles. This array contained 1,072 probes in which there were 571 related to 16S rRNA and 501 related to functional genes. Acid mine drainage (AMD) presents numerous problems to the aquatic life and surrounding ecosystems. However, little is known about the geographic distribution, diversity, composition, structure and function of AMD microbial communities. In this study, we analyzed the geographic distribution of AMD microbial communities from twenty sites using restriction fragment length polymorphism (RFLP) analysis of 16S rRNA genes, and the results showed that AMD microbial communities were geographically distributed and had high variations among different sites. Then an AMD-specific microarray was used to further analyze nine AMD microbial communities, and showed that those nine AMD microbial communities had high variations measured by the number of detected genes, overlapping genes between samples, unique genes, and diversity indices. Statistical analyses indicated that the concentrations of Fe, S, Ca, Mg, Zn, Cu and pH had strong impacts on both phylogenetic and functional diversity, composition, and structure of AMD microbial communities. This study provides insights into our understanding of the geographic distribution, diversity, composition, structure and functional potential of AMD microbial communities and key environmental factors shaping them. This study investigated the geographic distribution of Acid Mine Drainages microbial communities using a 16S rRNA gene-based RFLP method and the diversity, composition and structure of AMD microbial communities phylogenetically and functionally using an AMD-specific microarray which contained 1,072 probes ( 571 related to 16S rRNA and 501 related to functional genes). The functional genes in the microarray were involved in carbon metabolism (158), nitrogen metabolism (72), sulfur metabolism (39), iron metabolism (68), DNA replication and repair (97), metal-resistance (27), membrane-relate gene (16), transposon (13) and IST sequence (11).

Project description:Wastewater treatment plants use a variety of bioreactor types and configurations to remove organic matter and nutrients. Little is known regarding the effects of different configurations and within-plant immigration on microbial community dynamics. Previously, we found that the structure of ammonia-oxidizing bacterial (AOB) communities in a full-scale dispersed growth activated sludge bioreactor correlated strongly with levels of NO2- entering the reactor from an upstream trickling filter (Wells et al 2009). Here, to further examine this puzzling association, we profile within-plant microbial biogeography (spatial variation) and test the hypothesis that substantial microbial immigration occurs along a transect (raw influent, trickling filter biofilm, trickling filter effluent, and activated sludge) at the same full-scale wastewater treatment plant. AOB amoA gene abundance increased >30-fold between influent and trickling filter effluent concomitant with NO2- production, indicating unexpected growth and activity of AOB within the trickling filter. Nitrosomonas europaea was the dominant AOB phylotype in trickling filter biofilm and effluent, while a distinct ‘Nitrosomonas-like’ lineage dominated in activated sludge. Prior time series indicated that this ‘Nitrosomonas-like’ lineage was dominant when NO2- levels in the trickling filter effluent (i.e., activated sludge influent) were low, while N. europaea became dominant in the activated sludge when NO2- levels were high. This is consistent with the hypothesis that NO2- production may co-occur with biofilm sloughing, releasing N. europaea from the trickling filter into the activated sludge bioreactor. Phylogenetic microarray (PhyloChip) analyses revealed significant spatial variation in taxonomic diversity, including a large excess of methanogens in the trickling filter relative to activated sludge and attenuation of Enterobacteriaceae across the transect, and demonstrated transport of a highly diverse microbial community via the trickling filter effluent to the activated sludge bioreactor. Our results provide compelling evidence that substantial immigration between coupled process units occurs and may exert significant influence over microbial community dynamics within staged bioreactors.