Dataset Information

Integrated genomic and metabolomic profiling of ISC1, an emerging Leishmania donovani population in the Indian subcontinent

ABSTRACT:

Leishmania donovani is the responsible agent for visceral leishmaniasis (VL) in the Indian subcontinent (ISC). The disease is lethal without treatment and causes 0.2 to 0.4 million cases each year. Recently, reports of VL in Nepalese hilly districts have increased as well as VL cases caused by L. donovani from the ISC1 genetic group, a new and emerging genotype. In this study, we perform for the first time an integrated, untargeted genomics and metabolomics approach to characterize ISC1, in comparison with the Core Group (CG), main population that drove the most recent outbreak of VL in the ISC. We show that the ISC1 population is very different from the CG, both at genome and metabolome levels. The genomic differences include SNPs, CNV and small indels in genes coding for known virulence factors, immunogens and surface proteins. Both genomic and metabolic approaches highlighted dissimilarities related to membrane lipids, the nucleotide salvage pathway and the urea cycle in ISC1 versus CG. Many of these pathways and molecules are important for the interaction with the host/extracellular environment. Altogether, our data predict major functional differences in ISC1 versus CG parasites, including virulence. Therefore, particular attention is required to monitor the fate of this emerging ISC1 population in the ISC, especially in a post-VL elimination context.

Linked cross omic data sets:

Raw sequencing data associated with this study are available in the European Nucleotide Archive (ENA): accession number PRJNA419531.

INSTRUMENT(S): Liquid Chromatography MS - alternating - hilic

SUBMITTER: Bart Cuypers

PROVIDER: MTBLS1606 | MetaboLights | 2022-09-15

REPOSITORIES: MetaboLights

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Publications

Integrated genomic and metabolomic profiling of ISC1, an emerging Leishmania donovani population in the Indian subcontinent.

Cuypers Bart B Berg Maya M Imamura Hideo H Dumetz Franck F De Muylder Géraldine G Domagalska Malgorzata A MA Rijal Suman S Bhattarai Narayan Raj NR Maes Ilse I Sanders Mandy M Cotton James A JA Meysman Pieter P Laukens Kris K Dujardin Jean-Claude JC

Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases 20180419

Leishmania donovani is the responsible agent for visceral leishmaniasis (VL) in the Indian subcontinent (ISC). The disease is lethal without treatment and causes 0.2 to 0.4 million cases each year. Recently, reports of VL in Nepalese hilly districts have increased as well as VL cases caused by L. donovani from the ISC1 genetic group, a new and emerging genotype. In this study, we perform for the first time an integrated, untargeted genomics and metabolomics approach to characterize ISC1, in comp ...[more]

PMID: 29679745

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			Action	DRS
	MTBLS1606	Other
	FILES	Other
	a_MTBLS1606_LC-MS_alternating_hilic_metabolite_profiling.txt	Txt
	files-all.json	Other
	i_Investigation.txt	Txt