Dataset Information

Searching for metabolic changes in urine headspace composition as an effect of strenuous walking

ABSTRACT: The present investigation uses proton transfer reaction mass spectrometry (PTR-MS) combined with multivariate and univariate statistical analyses to study potential biomarkers for altered metabolism in urine due to strenuous walking. Urine samples, in concurrence with breath and blood samples, were taken from 51 participants (23 controls, 11 type-1 diabetes, 17 type-2 diabetes) during the Dutch endurance walking event, the International Four Days Marches. Multivariate analysis allowed for discrimination of before and after exercise for all three groups (control, type-1 and type-2 diabetes) and on three out of 4 days. The analysis highlighted 12 molecular ions contributing to this discrimination. Of these, acetic acid in urine is identified as a significant marker for exercise effects induced by walking; an increase is observed as an effect of walking. Analysis of acetone concentration with univariate tools resulted in different information when compared to breath as a function of exercise, revealing an interesting effect of time over the 4 days. In breath, acetone provides an immediate snapshot of metabolism, whereas urinary acetone will result from longer term diffusion processes, providing a time averaged view of metabolism. The potential to use PTR-MS measurements of urine to monitor exercise effects is exhibited, and may be utilized to monitor subjects in mass participation exercise events.

INSTRUMENT(S): PTR-MS Quad (Radboud)

SUBMITTER: Phil Brown

PROVIDER: MTBLS164 | MetaboLights | 2016-12-14

REPOSITORIES: MetaboLights

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Dataset's files

Source:

			Action	DRS
	MTBLS164	Other
	FILES	Other
	a_MTBLS164_vierdagse_urine_metabolite_profiling_mass_spectrometry.txt	Txt
	i_Investigation.txt	Txt
	m_MTBLS164_vierdagse_urine_metabolite_profiling_mass_spectrometry_v2_maf.tsv	Tabular

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Publications

Changes in urine headspace composition as an effect of strenuous walking.

Samudrala Devasena D Geurts Brigitte B Brown Phil A PA Szymańska Ewa E Mandon Julien J Jansen Jeroen J Buydens Lutgarde L Harren Frans J M FJ Cristescu Simona M SM

Metabolomics : Official journal of the Metabolomic Society 20150531 6

The present investigation uses proton transfer reaction mass spectrometry (PTR-MS) combined with multivariate and univariate statistical analyses to study potential biomarkers for altered metabolism in urine due to strenuous walking. Urine samples, in concurrence with breath and blood samples, were taken from 51 participants (23 controls, 11 type-1 diabetes, 17 type-2 diabetes) during the Dutch endurance walking event, the <i>International Four Days Marches</i>. Multivariate analysis allowed for ...[more]

PMID: 26491419

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