Dataset Information

Seagrasses excrete sugars to their rhizosphere making them the sweet spots in the sea (Plant roots; MALDI-MSI assay)

ABSTRACT:

Seagrasses are one of the most efficient natural sinks of carbon dioxide (CO2) on Earth. Despite covering less than 0.1% of coastal regions, they have the capacity to bury up to 10% of marine organic matter and can bury the same amount of carbon 35 times faster than tropical rainforests. On land, the soil’s ability to sequestrate carbon is intimately linked to microbial metabolism. Despite the growing attention to the link between plant production, microbial communities, and the carbon cycle in terrestrial ecosystems, these processes remain enigmatic in the sea. Here, we show that seagrasses excrete organic sugars, namely in the form of sucrose, into their rhizospheres. Surprisingly, the microbial communities living underneath meadows do not fully use this sugar stock in their metabolism. Instead, sucrose piles up in the sediments to mM concentrations underneath multiple types of seagrass meadows. Sediment incubation experiments show that microbial communities living underneath a meadow use sucrose at low metabolic rates. Our metagenomic analyses revealed that the distinct community of microorganisms occurring underneath meadows is limited in their ability to degrade simple sugars, which allows these compounds to persist in the environment over relatively long periods of time. Our findings reveal how seagrasses form blue carbon stocks despite the relatively small area they occupy. Unfortunately, anthropogenic disturbances are threatening the long-term persistence of seagrass meadows. Given that these sediments contain a large stock of sugars that heterotopic bacteria can degrade, it is even more important to protect these ecosystems from degradation.

MALDI-MSI assay of Plant roots is reported in the current study MTBLS1746

GC-MS assay of Seawater sediment is reported in MTBLS1570

GC-MS assay of Plant tissues is reported in MTBLS1579

GC-MS assay of Sediment porewater is reported in MTBLS1610

INSTRUMENT(S): MS Imaging -

SUBMITTER: Manuel Liebeke

PROVIDER: MTBLS1746 | MetaboLights | 2022-01-28

REPOSITORIES: MetaboLights

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Project description:Seagrasses are one of the most efficient natural sinks of carbon dioxide (CO2) on Earth. Despite covering less than 0.1% of coastal regions, they have the capacity to bury up to 10% of marine organic matter and can bury the same amount of carbon 35 times faster than tropical rainforests. On land, the soil’s ability to sequestrate carbon is intimately linked to microbial metabolism. Despite the growing attention to the link between plant production, microbial communities, and the carbon cycle in terrestrial ecosystems, these processes remain enigmatic in the sea. Here, we show that seagrasses excrete organic sugars, namely in the form of sucrose, into their rhizospheres. Surprisingly, the microbial communities living underneath meadows do not fully use this sugar stock in their metabolism. Instead, sucrose piles up in the sediments to mM concentrations underneath multiple types of seagrass meadows. Sediment incubation experiments show that microbial communities living underneath a meadow use sucrose at low metabolic rates. Our metagenomic analyses revealed that the distinct community of microorganisms occurring underneath meadows is limited in their ability to degrade simple sugars, which allows these compounds to persist in the environment over relatively long periods of time. Our findings reveal how seagrasses form blue carbon stocks despite the relatively small area they occupy. Unfortunately, anthropogenic disturbances are threatening the long-term persistence of seagrass meadows. Given that these sediments contain a large stock of sugars that heterotopic bacteria can degrade, it is even more important to protect these ecosystems from degradation. GC-MS assay of Sediment porewater is reported in the current study <a href='https://www.ebi.ac.uk/metabolights/MTBLS1610' rel='noopener noreferrer' target='_blank'>MTBLS1610</a>GC-MS assay of Seawater sediment is reported in <a href='https://www.ebi.ac.uk/metabolights/MTBLS1570' rel='noopener noreferrer' target='_blank'>MTBLS1570</a>GC-MS assay of Plant tissues is reported in <a href='https://www.ebi.ac.uk/metabolights/MTBLS1579' rel='noopener noreferrer' target='_blank'>MTBLS1579</a>MALDI-MSI assay of Plant roots is reported in the <a href='https://www.ebi.ac.uk/metabolights/MTBLS1746' rel='noopener noreferrer' target='_blank'>MTBLS1746</a>

Project description:Seagrasses are one of the most efficient natural sinks of carbon dioxide (CO2) on Earth. Despite covering less than 0.1% of coastal regions, they have the capacity to bury up to 10% of marine organic matter and can bury the same amount of carbon 35 times faster than tropical rainforests. On land, the soil’s ability to sequestrate carbon is intimately linked to microbial metabolism. Despite the growing attention to the link between plant production, microbial communities, and the carbon cycle in terrestrial ecosystems, these processes remain enigmatic in the sea. Here, we show that seagrasses excrete organic sugars, namely in the form of sucrose, into their rhizospheres. Surprisingly, the microbial communities living underneath meadows do not fully use this sugar stock in their metabolism. Instead, sucrose piles up in the sediments to mM concentrations underneath multiple types of seagrass meadows. Sediment incubation experiments show that microbial communities living underneath a meadow use sucrose at low metabolic rates. Our metagenomic analyses revealed that the distinct community of microorganisms occurring underneath meadows is limited in their ability to degrade simple sugars, which allows these compounds to persist in the environment over relatively long periods of time. Our findings reveal how seagrasses form blue carbon stocks despite the relatively small area they occupy. Unfortunately, anthropogenic disturbances are threatening the long-term persistence of seagrass meadows. Given that these sediments contain a large stock of sugars that heterotopic bacteria can degrade, it is even more important to protect these ecosystems from degradation. GC-MS assay of Plant tissues is reported in the current study <a href='https://www.ebi.ac.uk/metabolights/MTBLS1579' rel='noopener noreferrer' target='_blank'>MTBLS1579</a>GC-MS assay of Seawater sediment is reported in <a href='https://www.ebi.ac.uk/metabolights/MTBLS1570' rel='noopener noreferrer' target='_blank'>MTBLS1570</a>GC-MS assay of Sediment porewater is reported in <a href='https://www.ebi.ac.uk/metabolights/MTBLS1610' rel='noopener noreferrer' target='_blank'>MTBLS1610</a>MALDI-MSI assay of Plant roots is reported in the <a href='https://www.ebi.ac.uk/metabolights/MTBLS1746' rel='noopener noreferrer' target='_blank'>MTBLS1746</a>

Project description:Seagrasses are one of the most efficient natural sinks of carbon dioxide (CO2) on Earth. Despite covering less than 0.1% of coastal regions, they have the capacity to bury up to 10% of marine organic matter and can bury the same amount of carbon 35 times faster than tropical rainforests. On land, the soil’s ability to sequestrate carbon is intimately linked to microbial metabolism. Despite the growing attention to the link between plant production, microbial communities, and the carbon cycle in terrestrial ecosystems, these processes remain enigmatic in the sea. Here, we show that seagrasses excrete organic sugars, namely in the form of sucrose, into their rhizospheres. Surprisingly, the microbial communities living underneath meadows do not fully use this sugar stock in their metabolism. Instead, sucrose piles up in the sediments to mM concentrations underneath multiple types of seagrass meadows. Sediment incubation experiments show that microbial communities living underneath a meadow use sucrose at low metabolic rates. Our metagenomic analyses revealed that the distinct community of microorganisms occurring underneath meadows is limited in their ability to degrade simple sugars, which allows these compounds to persist in the environment over relatively long periods of time. Our findings reveal how seagrasses form blue carbon stocks despite the relatively small area they occupy. Unfortunately, anthropogenic disturbances are threatening the long-term persistence of seagrass meadows. Given that these sediments contain a large stock of sugars that heterotopic bacteria can degrade, it is even more important to protect these ecosystems from degradation. GC-MS assay of Seawater sediment is reported in the current study MTBLS1570GC-MS assay of Plant tissues is reported in <a href='https://www.ebi.ac.uk/metabolights/MTBLS1579' rel='noopener noreferrer' target='_blank'>MTBLS1579</a>GC-MS assay of Sediment porewater is reported in <a href='https://www.ebi.ac.uk/metabolights/MTBLS1610' rel='noopener noreferrer' target='_blank'>MTBLS1610</a>MALDI-MSI assay of Plant roots is reported in the <a href='https://www.ebi.ac.uk/metabolights/MTBLS1746' rel='noopener noreferrer' target='_blank'>MTBLS1746</a>

Project description:To effectively monitor microbial populations in acidic environments and bioleaching systems, a comprehensive 50-mer-based oligonucleotide microarray was developed based on most of the known genes associated with the acidophiles. This array contained 1,072 probes in which there were 571 related to 16S rRNA and 501 related to functional genes. Acid mine drainage (AMD) presents numerous problems to the aquatic life and surrounding ecosystems. However, little is known about the geographic distribution, diversity, composition, structure and function of AMD microbial communities. In this study, we analyzed the geographic distribution of AMD microbial communities from twenty sites using restriction fragment length polymorphism (RFLP) analysis of 16S rRNA genes, and the results showed that AMD microbial communities were geographically distributed and had high variations among different sites. Then an AMD-specific microarray was used to further analyze nine AMD microbial communities, and showed that those nine AMD microbial communities had high variations measured by the number of detected genes, overlapping genes between samples, unique genes, and diversity indices. Statistical analyses indicated that the concentrations of Fe, S, Ca, Mg, Zn, Cu and pH had strong impacts on both phylogenetic and functional diversity, composition, and structure of AMD microbial communities. This study provides insights into our understanding of the geographic distribution, diversity, composition, structure and functional potential of AMD microbial communities and key environmental factors shaping them. This study investigated the geographic distribution of Acid Mine Drainages microbial communities using a 16S rRNA gene-based RFLP method and the diversity, composition and structure of AMD microbial communities phylogenetically and functionally using an AMD-specific microarray which contained 1,072 probes ( 571 related to 16S rRNA and 501 related to functional genes). The functional genes in the microarray were involved in carbon metabolism (158), nitrogen metabolism (72), sulfur metabolism (39), iron metabolism (68), DNA replication and repair (97), metal-resistance (27), membrane-relate gene (16), transposon (13) and IST sequence (11).

			Action	DRS
	MTBLS1746	Other
	FILES	Other
	a_MTBLS1746_MSImaging___metabolite_profiling.txt	Txt
	files-all.json	Other
	i_Investigation.txt	Txt

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Seagrasses excrete sugars to their rhizosphere making them the sweet spots in the sea (Plant roots; MALDI-MSI assay)

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