Dataset Information

Comparative metabolomics analysis of bronchial epithelium during barrier establishment after allergen exposure

ABSTRACT:

BACKGROUND: Several studies have shown a correlation between an altered metabolome and respiratory allergies. The epithelial barrier hypothesis proposes that an epithelial barrier dysfunction can result in allergic diseases development. Der p 1 allergen from house dust mite is a renowned epithelial barrier disruptor and allergy initiator due to its cysteine-protease activity. Here, we compared the metabolic profile of the bronchial epithelium exposed or not to Der p 1 during barrier establishment to understand its active role in allergy development.

METHODS: Calu-3 cells were cultivated in air-liquid interface cultures and exposed to either Der p 1 or Ole e 1 allergens during barrier establishment. The comparative metabolomics analysis of apical and basolateral media were performed using liquid chromatography and capillary electrophoresis both coupled to mass spectrometry.

RESULTS: We showed that epithelial barrier disruption by Der p 1 was associated with a specific metabolic profile, which was highly dependent on the state of the epithelium at the time of contact. Moreover, an apical-basolateral distribution of the metabolites was also observed, indicating a compartmentalization of the response with differential metabolic patterns. A number of metabolites were changed by Der p 1, mainly related to amino acids metabolism, such as L-arginine, L-kynurenine and L-methionine.

CONCLUSION: This work is the first report on the metabolic response in human bronchial epithelial cells associated with cysteine-protease Der p 1 activity, which could contribute to allergy development. Moreover, it supports a reformulated epithelial barrier hypothesis that might help to explain allergies and their increasing prevalence.

INSTRUMENT(S): Liquid Chromatography MS - negative - reverse phase, Capillary electrophoresis MS - positive, Liquid Chromatography MS - positive - reverse phase

SUBMITTER: Juan Rodriguez-Coira

PROVIDER: MTBLS1755 | MetaboLights | 2023-08-22

REPOSITORIES: MetaboLights

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Dataset's files

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			Action	DRS
	MTBLS1755	Other
	FILES	Other
	a_MTBLS1755_CE-MS_positive__metabolite_profiling(1).txt	Txt
	a_MTBLS1755_CE-MS_positive__metabolite_profiling.txt	Txt
	a_MTBLS1755_LC-MS_negative_reverse-phase_metabolite_profiling.txt	Txt

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Publications

Comparative metabolomics analysis of bronchial epithelium during barrier establishment after allergen exposure.

López-Rodríguez Juan Carlos JC Rodríguez-Coira Juan J Benedé Sara S Barbas Coral C Barber Domingo D Villalba María Teresa MT Escribese María Marta MM Villaseñor Alma A Batanero Eva E

Clinical and translational allergy 20210901 7

<h4>Background</h4>Several studies have shown a correlation between an altered metabolome and respiratory allergies. The epithelial barrier hypothesis proposes that an epithelial barrier dysfunction can result in allergic diseases development. Der p 1 allergen from house dust mite is a renowned epithelial barrier disruptor and allergy initiator due to its cysteine-protease activity. Here, we compared the metabolic profile of the bronchial epithelium exposed or not to Der p 1 during barrier estab ...[more]

PMID: 34582104

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