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Mass spectrometry imaging of phosphatidylcholine metabolism in lungs administered with therapeutic surfactants and isotopic tracers

ABSTRACT:

Mass spectrometry imaging (MSI) visualises molecular distributions throughout tissues but is blind to dynamic metabolic processes. Here, MSI with high mass resolution together with multiple stable isotope labelling provided spatial analyses of phosphatidylcholine (PC) metabolism in mouse lungs. Dysregulated surfactant metabolism is central to many respiratory diseases. Metabolism and turnover of therapeutic pulmonary surfactants were imaged from distributions of intact and metabolic products of an added tracer, universally 13C-labelled dipalmitoyl PC (U[13C]DPPC). The parenchymal distributions of newly synthesised PC species were also imaged from incorporations of methyl-D9-choline. This dual labelling strategy demonstrated both lack of inhibition of endogenous PC synthesis by exogenous surfactant and location of acyl chain remodelling processes acting on the U[13C]DPPC-labelled surfactant, leading to formation of polyunsaturated PC lipids. This ability to visualise discrete metabolic events will greatly enhance our understanding of lipid metabolism in diverse tissues, and has potential application to both clinical and experimental studies.

INSTRUMENT(S): MS Imaging -

SUBMITTER: Shane Ellis

PROVIDER: MTBLS2075 | MetaboLights | 2020-10-30

REPOSITORIES: MetaboLights

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			Action	DRS
	MTBLS2075	Other
	FILES	Other
	a_MTBLS2075_MSImaging___metabolite_profiling.txt	Txt
	i_Investigation.txt	Txt
	m_MTBLS2075_MSImaging___metabolite_profiling_v2_maf.tsv	Tabular

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