Dataset Information

Microbial liberation of N-methylserotonin from orange fiber in gnotobiotic mice and humans

ABSTRACT: Plant fibers in byproduct streams produced by non-harsh food processing methods represent biorepositories of diverse, naturally occurring, and physiologically active biomolecules. To demonstrate one approach for their characterization, mass spectrometry of intestinal contents from gnotobiotic mice, plus in vitro studies, revealed liberation of N-methylserotonin from orange fibers by human gut microbiota members including Bacteroides ovatus. Functional genomic analyses of B. ovatus strains grown under permissive and non-permissive N-methylserotonin 'mining' conditions revealed polysaccharide utilization loci that target pectins whose expression correlate with strain-specific liberation of this compound. N-methylserotonin, orally administered to germ-free mice, reduced adiposity, altered liver glycogenesis, shortened gut transit time, and changed expression of genes that regulate circadian rhythm in the liver and colon. In human studies, dose-dependent, orange-fiber-specific fecal accumulation of N-methylserotonin positively correlated with levels of microbiome genes encoding enzymes that digest pectic glycans. Identifying this type of microbial mining activity has potential therapeutic implications.

INSTRUMENT(S): Liquid Chromatography MS - positive - reverse phase

SUBMITTER: JIYE CHENG

PROVIDER: MTBLS2331 | MetaboLights | 2022-07-28

REPOSITORIES: MetaboLights

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Microbial liberation of N-methylserotonin from orange fiber in gnotobiotic mice and humans.

Han Nathan D ND Cheng Jiye J Delannoy-Bruno Omar O Webber Daniel D Terrapon Nicolas N Henrissat Bernard B Rodionov Dmitry A DA Arzamasov Aleksandr A AA Osterman Andrei L AL Hayashi David K DK Meynier Alexandra A Vinoy Sophie S Desai Chandani C Marion Stacey S Barratt Michael J MJ Heath Andrew C AC Gordon Jeffrey I JI

Cell 20220627 14

Plant fibers in byproduct streams produced by non-harsh food processing methods represent biorepositories of diverse, naturally occurring, and physiologically active biomolecules. To demonstrate one approach for their characterization, mass spectrometry of intestinal contents from gnotobiotic mice, plus in vitro studies, revealed liberation of N-methylserotonin from orange fibers by human gut microbiota members including Bacteroides ovatus. Functional genomic analyses of B. ovatus strains grown ...[more]

PMID: 35764090

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			Action	DRS
	MTBLS2331	Other
	FILES	Other
	a_MTBLS2331_LC-MS_positive_reverse-phase_metabolite_profiling.txt	Txt
	files-all.json	Other
	i_Investigation.txt	Txt